25-Hydroxycholesterol

Name: 25-Hydroxycholesterol
Brand: Tocris Bioscience
SKU: 5741

Tocris Bioscience, part of Bio-Techne | Catalog # 5741

Citations (3)

COA / SDS

Inhibits hepatitis C viral life cycle; also α-crystallin (cryAB) chaperone

Key Product Details

Description:	Inhibits hepatitis C viral life cycle; also α-crystallin (cryAB) chaperone
Chemical Name:	Cholest-5-ene-3β,25-diol
Purity:	≥95% (HPLC)
Molecular Weight:	402.65

View Full Technical Details for 25-Hydroxycholesterol

Technical Data
Citations

Biological Activity for 25-Hydroxycholesterol

25-Hydroxycholesterol is an antiviral. Stimulates expression of miR-185 microRNA which inhibits the hepatitis C virus life cycle via inhibition of cholesterol biosynthesis and virus cell membrane fusion. Also α-crystallin (cryAB) chaperone (K_d = 10.1 μM). Blocks α-crystallin aggregation and partially reverses cataract formation by binding and stabilizing soluble forms of cryAB in vitro and in vivo.

Technical Data for 25-Hydroxycholesterol

M. Wt	402.65
Formula	C₂₇H₄₆O₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2140-46-7
PubChem ID	65094
InChi Key	INBGSXNNRGWLJU-ZHHJOTBYSA-N
Smiles	O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 25-Hydroxycholesterol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	8.05	20

Preparing Stock Solutions for 25-Hydroxycholesterol

The following data is based on the product molecular weight 402.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	12.42 mL	62.09 mL	124.18 mL
1 mM	2.48 mL	12.42 mL	24.84 mL
2 mM	1.24 mL	6.21 mL	12.42 mL
10 mM	0.25 mL	1.24 mL	2.48 mL

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Reconstitution Calculator

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References for 25-Hydroxycholesterol

References are publications that support the biological activity of the product.

Makley Pharmacological chaperone for alpha-crystallin partially restores transparency in cataract models. Science 2015 PMID: 26542570
Singaravelu MicroRNAs regulate the immunometabolic response to viral infection in the liver. Nat.Chem.Biol. 2015 PMID: 26479438