3PO

Name: 3PO
Brand: Tocris Bioscience
SKU: 5121

Tocris Bioscience, part of Bio-Techne | Catalog # 5121

Citations (3)

COA / SDS

PFKFB3 inhibitor

Key Product Details

Description:	PFKFB3 inhibitor
Chemical Name:	3-(3-Pyridinyl)-1-(4-pyridinyl)-2-propen-1-one
Purity:	≥99% (HPLC)
Molecular Weight:	210.23

View Full Technical Details for 3PO

Technical Data
Citations

Biological Activity for 3PO

3PO is originally reported to inhibit PFKFB3 (IC₅₀ = 25 μM). More recent reports failed to demonstrate activity in a PFKFB3 kinase assay. Reduces glycolytic flux and suppresses glucose uptake. Inhibits endothelial cell proliferation and causes G₂/M cell cycle arrest in vitro. Attenuates vessel sprouting and tumor growth in vivo. Amplifies the antiangiogenic effect of VEGFR blockade.

Technical Data for 3PO

M. Wt	210.23
Formula	C₁₃H₁₀N₂O
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	13309-08-5
PubChem ID	5720233
InChi Key	UOWGYMNWMDNSTL-ONEGZZNKSA-N
Smiles	O=C(/C=C/C2=CN=CC=C2)C1=CC=NC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3PO

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	21.02	100

Preparing Stock Solutions for 3PO

The following data is based on the product molecular weight 210.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.76 mL	23.78 mL	47.57 mL
5 mM	0.95 mL	4.76 mL	9.51 mL
10 mM	0.48 mL	2.38 mL	4.76 mL
50 mM	0.10 mL	0.48 mL	0.95 mL

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References for 3PO

References are publications that support the biological activity of the product.

Schoors Partial and transient reduction of glycolysis by PFKFB3 blockade reduces pathological angiogenesis. Cell Metab. 2014 PMID: 24332967
Boyd Structure-based design of potent and selective inhibitors of the metabolic kinase PFKFB3. J.Med.Chem. 2015 PMID: 25849762
Clem Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth. Mol.Cancer Ther. 2008 PMID: 18202014

3PO