Ac₄ManNDAz

Name: Ac 4 ManNDAz
Brand: Tocris Bioscience
SKU: 7553

Tocris Bioscience, part of Bio-Techne | Catalog # 7553

COA / SDS

Photo-crosslinking probe for studying glycoproteins and glycolipids

Key Product Details

Description:	Photo-crosslinking probe for studying glycoproteins and glycolipids
Chemical Name:	2-Deoxy-2-[[3-(3-methyl-3H-diazirin-3-yl)-1-oxopropyl]amino]-D-mannopyranose-1,3,4,6-tetraacetate
Purity:	≥98% (HPLC)
Molecular Weight:	457.44

View Full Technical Details for Ac₄ManNDAz

Technical Data
Citations

Biological Activity for Ac₄ManNDAz

Ac₄ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac₄ManNDAz is metabolized and incorporated into cell surface glycoproteins as a photo-crosslinking sialic acid analog. Upon UV irradiation, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby molecules.

Technical Data for Ac₄ManNDAz

M. Wt	457.44
Formula	C₁₉H₂₇N₃O₁₀
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1015698-39-1
PubChem ID	122400676
InChi Key	UZYLHDJBFVVKBM-GAMQSXCYSA-N
Smiles	CC(OC[C@H]1OC([C@H]([C@H]([C@@H]1OC(C)=O)OC(C)=O)NC(CCC2(N=N2)C)=O)OC(C)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ac₄ManNDAz

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	45.74	100
	DMSO	45.74	100

Preparing Stock Solutions for Ac₄ManNDAz

The following data is based on the product molecular weight 457.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.19 mL	10.93 mL	21.86 mL
5 mM	0.44 mL	2.19 mL	4.37 mL
10 mM	0.22 mL	1.09 mL	2.19 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for Ac₄ManNDAz

References are publications that support the biological activity of the product.

Cervin GM1 ganglioside-independent intoxication by Cholera toxin. PLoS.Pathog. 2018 PMID: 29432456
Bond Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars. Nat.Protoc. 2009 PMID: 19536272
Bond Metabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides. Bioconjug.Chem. 2011 PMID: 21838313
Tanaka Photoactivatable crosslinking sugars for capturing glycoprotein interactions. J.Am.Chem.Soc. 2008 PMID: 18293988

Ac₄ManNDAz

Tocris Bioscience, part of Bio-Techne | Catalog # 7553

Key Product Details

Biological Activity for Ac₄ManNDAz

Technical Data for Ac₄ManNDAz

Solubility for Ac₄ManNDAz

Preparing Stock Solutions for Ac₄ManNDAz

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Ac₄ManNDAz

Product Documents for Ac₄ManNDAz

COA

Certificate of Analysis

SDS

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Ac4ManNDAz

Tocris Bioscience, part of Bio-Techne | Catalog # 7553

Key Product Details

Biological Activity for Ac4ManNDAz

Technical Data for Ac4ManNDAz

Solubility for Ac4ManNDAz

Preparing Stock Solutions for Ac4ManNDAz

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Ac4ManNDAz

Product Documents for Ac4ManNDAz

COA

Certificate of Analysis

SDS

Ac₄ManNDAz

Biological Activity for Ac₄ManNDAz

Technical Data for Ac₄ManNDAz

Solubility for Ac₄ManNDAz

Preparing Stock Solutions for Ac₄ManNDAz

References for Ac₄ManNDAz

Product Documents for Ac₄ManNDAz