AF 64394

Name: AF 64394
Brand: Tocris Bioscience
SKU: 6931

Tocris Bioscience, part of Bio-Techne | Catalog # 6931

COA / SDS

Potent and selective GPR3 inverse agonist

Key Product Details

Description:	Potent and selective GPR3 inverse agonist
Chemical Name:	N-[[4-Chloro-2-(1-methylethoxy)phenyl]methyl]-5-phenyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Purity:	≥98% (HPLC)
Molecular Weight:	393.87

View Full Technical Details for AF 64394

Technical Data
Citations

Biological Activity for AF 64394

AF 64394 is a potent and selective GPR3 inverse agonist (pIC₅₀ = 7.3). Selective for GPR3 over GPR6 and GPR12 (pIC₅₀ values are 5.1 and 4.9, respectively).

Compound Libraries for AF 64394

AF 64394 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for AF 64394

M. Wt	393.87
Formula	C₂₁H₂₀ClN₅O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1637300-25-4
PubChem ID	125286314
InChi Key	WBYNZQXAAWPAGR-UHFFFAOYSA-N
Smiles	CC(C)OC1=C(CNC2=CC(=NC3=NC=NN23)C2=CC=CC=C2)C=CC(Cl)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AF 64394

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	39.39	100

Preparing Stock Solutions for AF 64394

The following data is based on the product molecular weight 393.87.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.54 mL	12.69 mL	25.39 mL
5 mM	0.51 mL	2.54 mL	5.08 mL
10 mM	0.25 mL	1.27 mL	2.54 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

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Reconstitution Calculator

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References for AF 64394

References are publications that support the biological activity of the product.

Godlewski Mice lacking GPR3 receptors display late-onset obese phenotype due to impaired thermogenic function in brown adipose tissue. Sci.Rep. 2015 PMID: 26455425
Jensen The identification of GPR3 inverse agonist AF64394; the first small molecule inhibitor of GPR3 receptor function. Bioorg.Med.Chem.Lett. 2014 PMID: 25442311