AF-DX 384

Name: AF-DX 384
Brand: Tocris Bioscience
SKU: 1345

Tocris Bioscience, part of Bio-Techne | Catalog # 1345

Citations (7)

COA / SDS

Potent M2/M4 antagonist

Key Product Details

Description:	Potent M₂/M₄ antagonist
Chemical Name:	N-[2-[2-[(Dipropylamino)methyl]-1-piperidinyl]ethyl]-5,6-dihydro-6-oxo-11H-pyrido[2,3-b][1,4]benzodiazepine-11-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	478.64

View Full Technical Details for AF-DX 384

Technical Data
Citations

Biological Activity for AF-DX 384

AF-DX 384 is a potent M₂/M₄ selective antagonist (pK_i values are 8.22, 8.00, 7.51, 7.18 and 6.27 at human M₂, M₄, M₁, M₃ and M₅ receptors respectively).

Technical Data for AF-DX 384

M. Wt	478.64
Formula	C₂₇H₃₈N₆O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	118290-26-9
PubChem ID	119357
InChi Key	MZDYABXXPZNUCT-UHFFFAOYSA-N
Smiles	CCCN(CCC)CC1CCCCN1CCNC(=O)N1C2=C(C=CC=C2)C(=O)NC2=C1N=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AF-DX 384

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	23.93	50
	ethanol	4.79	10

Preparing Stock Solutions for AF-DX 384

The following data is based on the product molecular weight 478.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.18 mL	20.89 mL	41.79 mL
2.5 mM	0.84 mL	4.18 mL	8.36 mL
5 mM	0.42 mL	2.09 mL	4.18 mL
25 mM	0.08 mL	0.42 mL	0.84 mL

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References for AF-DX 384

References are publications that support the biological activity of the product.

Grailhe Regional changes in the cholinergic system in mice lacking monoamine A. Brain Res.Bull. 2009 PMID: 19111597
Miller Binding of [³H]AF-DX 384 to cloned and native muscarinic receptors. J.Pharmacol.Exp.Ther. 1991 PMID: 1941609
Dopke AF-DX 384 binding in rabbit cingulate cortex: two site kinetics and section autoradiography. J.Pharmacol.Exp.Ther. 1995 PMID: 7616446