Apoptosis Activator 2

Name: Apoptosis Activator 2
Brand: Tocris Bioscience
SKU: 2098
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2098

(1)

Citations (5)

COA / SDS

Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. Selectively induces tumor cell apoptosis

Key Product Details

Description:	Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. Selectively induces tumor cell apoptosis
Chemical Name:	1-[(3,4-Dichlorophenyl)methyl]-1H-indole-2,3-dione
Purity:	≥99% (HPLC)
Molecular Weight:	306.14

View Full Technical Details for Apoptosis Activator 2

Technical Data
Citations / Reviews (1)

Biological Activity for Apoptosis Activator 2

Apoptosis Activator 2 is an apoptosis activator; promotes the cytochrome c-dependent oligomerization of Apaf-1 into the mature apoptosome. Increases procaspase-9 processing and subsequent caspase-3 activation. Induces apoptosis in tumor cells (IC₅₀ = 4 - 9 μM for leukemia cells) with weak or no effect on normal cell lines or those defective/deficient in Apaf-1, caspase-9 or caspase-3 activity (IC₅₀ > 40 μM).

Technical Data for Apoptosis Activator 2

M. Wt	306.14
Formula	C₁₅H₉Cl₂NO₂
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	79183-19-0
PubChem ID	1901244
InChi Key	KGRJPLRFGLMQMV-UHFFFAOYSA-N
Smiles	ClC1=CC=C(CN2C(=O)C(=O)C3=CC=CC=C23)C=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Apoptosis Activator 2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	1.53	5
	DMSO	30.61	100

Preparing Stock Solutions for Apoptosis Activator 2

The following data is based on the product molecular weight 306.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.27 mL	16.33 mL	32.66 mL
5 mM	0.65 mL	3.27 mL	6.53 mL
10 mM	0.33 mL	1.63 mL	3.27 mL
50 mM	0.07 mL	0.33 mL	0.65 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Apoptosis Activator 2

References are publications that support the biological activity of the product.

Jayaraman and Pike Progesterone attenuates oestrogen neuroprotection via downregulation of oestrogen receptor expression in cultured neurones. J.Neuroendocrinol. 2009 PMID: 19094096
Nguyen and Wells Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc.Natl.Acad.Sci.USA
Alavian Elevtaed p75NTR expression causes death of engrailed-deficient midbrain DArgic neurons by Erk1/2 suppression. Neural Dev. 2009 PMID: 19291307