BI 4394

Name: BI 4394
Brand: Tocris Bioscience
SKU: 8842

Tocris Bioscience, part of Bio-Techne | Catalog # 8842

COA / SDS

Highly potent and selective MMP-13 inhibitor

Key Product Details

Description:	Highly potent and selective MMP-13 inhibitor
Chemical Name:	2-Ethyl 5-[3-[[[(4-carboxyphenyl)methyl]amino]carbonyl]-1-methyl-1H-pyrazol-5-yl]-1H-indole-2-carboxylate
Purity:	≥98% (HPLC)
Molecular Weight:	446.46

View Full Technical Details for BI 4394

Technical Data
Citations

Biological Activity for BI 4394

BI 4394 is a highly potent and selective MMP-13 inhibitor (IC₅₀ = 1 nM). BI 4394 inhibits the degradation of isolated bovine nasal cartilage explants (IC₅₀ = 31 nM). It shows high selectivity (>1000 fold) for MMP-13 over other MMPs. In vivo, BI 4394 decreases the mean arthritic score vs control in a murine model of rheumatoid arthritis, when administered at a dose of 100 mg/kg over a 14-day period. BI 4394 is orally bioavailable.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the BI 4394 page on the SGC website.

Technical Data for BI 4394

M. Wt	446.46
Formula	C₂₄H₂₂N₄O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1222173-37-6
PubChem ID	45376872
InChi Key	MMJPVSDTLGFIQW-UHFFFAOYSA-N
Smiles	O=C(O)C1=CC=C(C=C1)CNC(=O)C2=NN(C(=C2)C=3C=CC=4NC(=CC4C3)C(=O)OCC)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BI 4394

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	44.65	100

Preparing Stock Solutions for BI 4394

The following data is based on the product molecular weight 446.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.24 mL	11.20 mL	22.40 mL
5 mM	0.45 mL	2.24 mL	4.48 mL
10 mM	0.22 mL	1.12 mL	2.24 mL
50 mM	0.04 mL	0.22 mL	0.45 mL

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References for BI 4394

References are publications that support the biological activity of the product.

Taylor Indole inhibitors of MMP-13 for arthritic disorders. ACS Omega 2021 PMID: 34337203
Taylor Fragment-based discovery of indole inhibitors of matrix metalloproteinase-13. J.Med.Chem. 2011 PMID: 22017539