BI 9321

Name: BI 9321
Brand: Tocris Bioscience
SKU: 6665

Tocris Bioscience, part of Bio-Techne | Catalog # 6665

COA / SDS

Nuclear receptor-binding SET domain (NSD) 3 antagonist; selectively binds PWWP1 domain

Key Product Details

Description:	Nuclear receptor-binding SET domain (NSD) 3 antagonist; selectively binds PWWP1 domain
Chemical Name:	(4-(5-(7-Fluoroquinolin-4-yl)-1-methyl-1H-imidazol-4-yl)-3,5-dimethylphenyl)methanamine trihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	469.81

View Full Technical Details for BI 9321

Technical Data
Citations

Biological Activity for BI 9321

BI 9321 is a nuclear receptor-binding SET domain (NSD) 3 antagonist (IC₅₀ = 1.2 μM). Selectively binds the PWWP1 domain of NSD3 (K_d = 166 nM). Antagonizes the interaction of H3 with NSD3-PWWP1 in U2OS cells.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the BI 9321 probe summary on the SGC website.

Technical Data for BI 9321

M. Wt	469.81
Formula	C₂₂H₂₁FN₄.3HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	2387510-87-2
InChi Key	DCDXMBVQCRHZMW-UHFFFAOYSA-N
Smiles	CN1C=NC(C2=C(C=C(C=C2C)CN)C)=C1C3=C4C=CC(F)=CC4=NC=C3.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BI 9321

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	46.98	100
	DMSO	46.98	100

Preparing Stock Solutions for BI 9321

The following data is based on the product molecular weight 469.81.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.13 mL	10.64 mL	21.29 mL
5 mM	0.43 mL	2.13 mL	4.26 mL
10 mM	0.21 mL	1.06 mL	2.13 mL
50 mM	0.04 mL	0.21 mL	0.43 mL

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References for BI 9321

References are publications that support the biological activity of the product.

Böttcher Fragment-based discovery of a chemical probe for the PWWP1 domain of NSD3. Nat.Chem.Biol. 2019 PMID: 31285596