BIO

Name: BIO
Brand: Tocris Bioscience
SKU: 3194

Tocris Bioscience, part of Bio-Techne | Catalog # 3194

Citations (52)

COA / SDS

Potent GSK-3 inhibitor; also inhibits cdks

Key Product Details

Description:	Potent GSK-3 inhibitor; also inhibits cdks
Alternative Names:	6BIO
Chemical Name:	(2'Z,3'E)-6-Bromoindirubin-3'-oxime
Purity:	≥98% (HPLC)
Molecular Weight:	356.17

View Full Technical Details for BIO

Technical Data
Citations

Biological Activity for BIO

BIO is a potent ATP-competitive glycogen synthase kinase-3 (GSK-3) inhibitor (IC₅₀ = 5 nM). Also inhibits cyclin-dependent kinases (cdks; IC₅₀ values are <100 nM for cdk2 and 5 and sub micromolar for cdk1, cdk4 and cdk9). Maintains self-renewal and pluripotency in embryonic stem cells via activation of the Wnt signaling pathway in vitro; also promotes proliferation and dedifferentiation in cardiomyocytes.

Negative Control also available.

Technical Data for BIO

M. Wt	356.17
Formula	C₁₆H₁₀BrN₃O₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	667463-62-9
PubChem ID	5287844
InChi Key	WNWSUJQVZJJGLF-SQFISAMPSA-N
Smiles	O/N=C(C1=CC=CC=C1N2)/C2=C3/C(NC4=C3C=CC(Br)=C4)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BIO

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	3.56	10
	ethanol	3.56	10

Preparing Stock Solutions for BIO

The following data is based on the product molecular weight 356.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	28.08 mL	140.38 mL	280.76 mL
0.5 mM	5.62 mL	28.08 mL	56.15 mL
1 mM	2.81 mL	14.04 mL	28.08 mL
5 mM	0.56 mL	2.81 mL	5.62 mL

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Reconstitution Calculator

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References for BIO

References are publications that support the biological activity of the product.

Jorda How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 2018 PMID: 30234987
Polychronopoulos Structural basis for the synthesis of indirubins as potent and selective inhibtors of glycogen synthase kinase-3 and cyclin dependent kinases. J.Med.Chem. 2004 PMID: 14761195
Tseng The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes. Chem.Biol. 2006 PMID: 16984885
Meijer GSK-3-selective inhibitors derived from tyrian purple indirubins. Chem.Biol. 2003 PMID: 14700633

BIO