CH 223191

Name: CH 223191
Brand: Tocris Bioscience
SKU: 3858
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3858

Citations (47)

COA / SDS

Potent aryl hydrocarbon receptor (AhR) antagonist

Key Product Details

Description:	Potent aryl hydrocarbon receptor (AhR) antagonist
Chemical Name:	1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl-1H-pyrazole-5-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	333.39

View Full Technical Details for CH 223191

Technical Data
Citations

Biological Activity for CH 223191

CH 223191 is a potent aryl hydrocarbon receptor (AhR) antagonist (IC₅₀ = 30 nM). Exhibits no AhR agonist-like activity (at concentrations up to 100 μM). Inhibits 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR-dependent transcription in vitro and reduces TCDD-induced toxicity in vivo. Attenuates Th17 differentiation of naive CD4 T cells and promotes expansion of hematopoietic stem cells in vitro.

Compound Libraries for CH 223191

CH 223191 is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for CH 223191

M. Wt	333.39
Formula	C₁₉H₁₉N₅O
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	301326-22-7
PubChem ID	3091786
InChi Key	LKTNEXPODAWWFM-UHFFFAOYSA-N
Smiles	CC1=C(/N=N/C2=CC=C(NC(C3=CC=NN3C)=O)C(C)=C2)C=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CH 223191

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	33.34	100
	ethanol	3.33	10

Preparing Stock Solutions for CH 223191

The following data is based on the product molecular weight 333.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.00 mL	15.00 mL	29.99 mL
5 mM	0.60 mL	3.00 mL	6.00 mL
10 mM	0.30 mL	1.50 mL	3.00 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

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References for CH 223191

References are publications that support the biological activity of the product.

Boitano Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science 2010 PMID: 20688981
Veldhoen Natural agonists for aryl hydrocarbon receptor in culture medium are essential for optimal differentiation of TH17 T cells. J.Exp.Med. 2009 PMID: 19114668
Dubrovska CXCR4 activation maintains a stem cell population in tamoxifen-resistant breast cancer cells through AhR signalling. Br.J.Cancer. 2012 PMID: 22644306
Kim Novel compound 2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) prevents 2,3,7,8-TCDD-induced toxicity by antagonizing the aryl hydrocarbon receptor. Mol.Pharmacol. 2006 PMID: 16540597