Clofarabine

Name: Clofarabine
Brand: Tocris Bioscience
SKU: 2600

Tocris Bioscience, part of Bio-Techne | Catalog # 2600

Citations (3)

COA / SDS

Deoxycytidine kinase (dCK) substrate

Key Product Details

Description:	Deoxycytidine kinase (dCK) substrate
Chemical Name:	2-Chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-9H-purin-6-amine
Purity:	≥99% (HPLC)
Molecular Weight:	303.68

View Full Technical Details for Clofarabine

Technical Data
Citations

Biological Activity for Clofarabine

Clofarabine is a deoxycytidine kinase (dCK) substrate. Phosphorylated to form clofarabine triphosphate, which competes with dATP for DNA polymerase-α and -ε and potently inhibits ribonucleotide reductase (IC₅₀ = 65 nM). Induces apoptosis by directly altering mitochondrial transmembrane potential. Demonstrates growth inhibition and cytotoxic activity in a variety of leukemias and solid tumors.

Compound Libraries for Clofarabine

Clofarabine is also offered as part of the Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Clofarabine

M. Wt	303.68
Formula	C₁₀H₁₁ClFN₅O₃
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	123318-82-1
PubChem ID	119182
InChi Key	WDDPHFBMKLOVOX-AYQXTPAHSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N(C=N3)C2=C3C(N)=NC(Cl)=N2)[C@H]1F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Clofarabine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.37	100

Preparing Stock Solutions for Clofarabine

The following data is based on the product molecular weight 303.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.29 mL	16.46 mL	32.93 mL
5 mM	0.66 mL	3.29 mL	6.59 mL
10 mM	0.33 mL	1.65 mL	3.29 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

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References for Clofarabine

References are publications that support the biological activity of the product.

Bonate Discovery and development of clofarabine: a nucleoside analogue for treating cancer. Nat.Rev.Drug Discov. 2006 PMID: 17016426
Carson Oral antilymphocyte activity and induction of apoptosis by 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine. Proc.Natl.Acad.Sci.USA
Parker Effects of 2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl) adenine on K562 cellular metabolism and the inhibition of human ribonucleotide reductase and DNA polymerases by its 5'-triphosphate. Cancer Res. 1991 PMID: 1707752