CRANAD 2

Name: CRANAD 2
Brand: Tocris Bioscience
SKU: 4803

Tocris Bioscience, part of Bio-Techne | Catalog # 4803

COA / SDS

Near-infrared probe that detects Aβ40 aggregates. Used for: amyloid-β detection in vitro and in vivo. Application: fluorescence microscopy, epifluorescence microscopy

Key Product Details

Description:	Near-infrared probe that detects Aβ40 aggregates. Used for: amyloid-β detection in vitro and in vivo. Application: fluorescence microscopy, epifluorescence microscopy
Chemical Name:	(T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3,5-dionato-kO³,kO⁵]difluoroboron
Purity:	≥98% (HPLC)
Molecular Weight:	410.26

View Full Technical Details for CRANAD 2

Technical Data
Citations

Biological Activity for CRANAD 2

Key information: CRANAD 2 is a curcumin derivatized near-infrared probe that binds to Aβ40 aggregates. Penetrates the blood-brain barrier.

Used for: amyloid- detection in vitro, tissue and in vivo. Shown to bind to plaques in APP-PS1 transgenic mice, in vitro. Detects senile plaques in 19-month-old Tg2576 mice in vivo.

Application: Fluorescence microscopy, epifluorescence microscopy.

Properties and Photophysical Data: CRANAD 2 binds to Aβ40 aggregates (K_d = 38 nM) and elicits upon interacting with Aβ aggregates, an emission blue shift (from 805 nm to 715 nm). When unbound in PBS, excitation and emission maxima (λ) are 640 nm and 805 nm, respectively.

Technical Data for CRANAD 2

M. Wt	410.26
Formula	C₂₃H₂₅BF₂N₂O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1193447-34-5
PubChem ID	46861929
InChi Key	GNLVJIICVWDSNI-LFYBBSHMSA-N
Smiles	CN(C)C(C=C3)=CC=C3/C=C/C1=CC(/C=C/C2=CC=C(N(C)C)C=C2)=[O+][B-](F)(F)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CRANAD 2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.05	5 with gentle warming

Preparing Stock Solutions for CRANAD 2

The following data is based on the product molecular weight 410.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	48.75 mL	243.75 mL	487.50 mL
0.25 mM	9.75 mL	48.75 mL	97.50 mL
0.5 mM	4.87 mL	24.37 mL	48.75 mL
2.5 mM	0.97 mL	4.87 mL	9.75 mL

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References for CRANAD 2

References are publications that support the biological activity of the product.

Ran Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species. PLoS One. 2011 PMID: 21559413
Ni In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice. Photoacoustics 2021 PMID: 34354924
Ran Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. J.Am.Chem.Soc. 2009 PMID: 19807070