Cyclophosphamide

Name: Cyclophosphamide
Brand: Tocris Bioscience
SKU: 4091

Tocris Bioscience, part of Bio-Techne | Catalog # 4091

Citations (4)

COA / SDS

Alkylating agent; chemotherapeutic

Key Product Details

Description:	Alkylating agent; chemotherapeutic
Chemical Name:	2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide
Purity:	≥99% (HPLC)
Molecular Weight:	261.09

View Full Technical Details for Cyclophosphamide

Technical Data
Citations

Biological Activity for Cyclophosphamide

Cyclophosphamide is a nitrogen mustard alkylating agent and prodrug. Phosphoramide mustard (active metabolite) forms DNA cross-links leading to cell death. Inhibits aldehyde dehydrogenase 1 (ALDH1) through its degradation product acrolein. Chemotherapeutic for the treatment of breast cancer; regulates Bax and Bcl-2 expression when administered with etoposide (Cat. No. 1226) in breast cancer cell lines.

Technical Data for Cyclophosphamide

M. Wt	261.09
Formula	C₇H₁₅Cl₂N₂O₂P
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	50-18-0
PubChem ID	2907
InChi Key	CMSMOCZEIVJLDB-UHFFFAOYSA-N
Smiles	O=P1(N(CCCl)CCCl)OCCCN1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyclophosphamide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	26.11	100
	DMSO	26.11	100

Preparing Stock Solutions for Cyclophosphamide

The following data is based on the product molecular weight 261.09.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.83 mL	19.15 mL	38.30 mL
5 mM	0.77 mL	3.83 mL	7.66 mL
10 mM	0.38 mL	1.92 mL	3.83 mL
50 mM	0.08 mL	0.38 mL	0.77 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Cyclophosphamide

References are publications that support the biological activity of the product.

Hengstler DNA strand breaks and DNA cross-links in peripheral mononuclear blood cells of ovarian cancer patients during chemotherapy with cyclophosphamide/carboplatin. Cancer Res.
Ren Inhibition of human aldehyde dehydrogenase 1 by the 4-hydroxycyclophosphamide degradation product acrolein. Drug Metab.Disp.
Gibson Regulation of BAX and BCL-2 expression in breast cancer cells by chemotherapy. Breast Cancer Research and Treatment 1999 PMID: 10481938

Product Documents for Cyclophosphamide

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Cyclophosphamide (anhydrous), which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov