Cysmethynil

Name: Cysmethynil
Brand: Tocris Bioscience
SKU: 7337

Tocris Bioscience, part of Bio-Techne | Catalog # 7337

COA / SDS

ICMT inhibitor; arrests cell-cycle at G1 phase

Key Product Details

Description:	ICMT inhibitor; arrests cell-cycle at G₁ phase
Chemical Name:	5-(3-Methylphenyl)-1-octyl-1H-indole-3-acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	376.54

View Full Technical Details for Cysmethynil

Technical Data
Citations

Biological Activity for Cysmethynil

Cysmethynil is an isoprenylcysteine carboxylmethyltransferase (ICMT) inhibitor (IC₅₀ = 2.4 μM). Cysmethynil reduces growth of RAS-mutant cell lines in vitro and in vivo (EC₅₀ = 20 μM) and reduces cell viability in a variety of cell lines (IC₅₀ = 16.8-23.3 μM). Tumor growth, mTOR signaling and cell proliferation are inhibited by cysmethynil; apoptosis is induced. Cysmethynil increases p27 and p21 proteins and arrests cell-cycle at the G₁ phase.

Technical Data for Cysmethynil

M. Wt	376.54
Formula	C₂₅H₃₂N₂O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	851636-83-4
PubChem ID	6918831
InChi Key	QIXBOOVPFRZHQQ-UHFFFAOYSA-N
Smiles	CCCCCCCCN1C=C(C2=CC(C3=CC(C)=CC=C3)=CC=C12)CC(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cysmethynil

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	37.65	100
	DMSO	18.83	50

Preparing Stock Solutions for Cysmethynil

The following data is based on the product molecular weight 376.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.66 mL	13.28 mL	26.56 mL
5 mM	0.53 mL	2.66 mL	5.31 mL
10 mM	0.27 mL	1.33 mL	2.66 mL
50 mM	0.05 mL	0.27 mL	0.53 mL

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References for Cysmethynil

References are publications that support the biological activity of the product.

Manu Inhibition of isoprenylcysteine carboxylmethyltransferase induces cell-cycle arrest and apoptosis through p21 and p21-regulated BNIP3 induction in pancreatic cancer. Mol.Cancer Ther. 2017 PMID: 28167504
Moore RAS-targeted therapies: is the undruggable drugged? Nat.Rev.Drug Discov. 2020 PMID: 32528145