Decitabine

Name: Decitabine
Brand: Tocris Bioscience
SKU: 2624
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2624

(1)

Citations (27)

COA / SDS

DNA methyltransferase inhibitor

Key Product Details

Description:	DNA methyltransferase inhibitor
Alternative Names:	2'-Deoxy-5-azacytidine,5-Aza-2'-deoxycytidine,NSC 127716
Chemical Name:	4-Amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,3,5-triazin-2(1H)-one
Purity:	≥99% (HPLC)
Molecular Weight:	228.21

View Full Technical Details for Decitabine

Technical Data
Citations / Reviews (1)

Biological Activity for Decitabine

Decitabine is a cytosine analog that once incorporated into DNA acts as a suicide substrate for DNA methyltransferase. Inhibits DNA methyltransferase and results in DNA hypomethylation and activation of silent genes. Chemotherapeutic agent; suppresses growth of human tumor cell lines. Demethylates differentiation-related genes; reverses embryonic stem cell differentiation.

For more information about how Decitabine may be used, see our protocol:

Highly Efficient Generation of CiPSCs from MEFs

Compound Libraries for Decitabine

Decitabine is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library, Tocriscreen FDA-Approved Drugs and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Decitabine

M. Wt	228.21
Formula	C₈H₁₂N₄O₄
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	2353-33-5
PubChem ID	451668
InChi Key	XAUDJQYHKZQPEU-KVQBGUIXSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(N)=NC2=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Decitabine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	11.41	50
	DMSO	11.41	50

Preparing Stock Solutions for Decitabine

The following data is based on the product molecular weight 228.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	8.76 mL	43.82 mL	87.64 mL
2.5 mM	1.75 mL	8.76 mL	17.53 mL
5 mM	0.88 mL	4.38 mL	8.76 mL
25 mM	0.18 mL	0.88 mL	1.75 mL

Molarity Calculator

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Reconstitution Calculator

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References for Decitabine

References are publications that support the biological activity of the product.

Momparler Pharmacology of 5-aza-2'-deoxycytidine (decitabine). Semin.Hematol. 2005 PMID: 16015507
Jackson-Grusby Mutagenicity of 5-aza-2'-deoxycytidine is mediated by the mammalian DNA methyltransferase. Proc.Natl.Acad.Sci.USA
Bender Inhibition of DNA methylation by 5-aza-2'-deoxycytidine supresses the growth of human tumour cell lines. Cancer Res. 1998 PMID: 9426064