Dihydrokainic acid

Name: Dihydrokainic acid
Brand: Tocris Bioscience
SKU: 0111
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 0111

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Citations (73)

COA / SDS

EAAT2 (GLT-1)-selective non-transportable inhibitor of L-glutamate and L-aspartate uptake

Key Product Details

Description:	EAAT2 (GLT-1)-selective non-transportable inhibitor of L-glutamate and L-aspartate uptake
Chemical Name:	(2S,3S,4R)-2-Carboxy-4-isopropyl-3-pyrrolidineacetic acid
Molecular Weight:	215.25

View Full Technical Details for Dihydrokainic acid

Technical Data
Citations / Reviews (1)

Biological Activity for Dihydrokainic acid

Dihydrokainic acid is a selective EAAT2(GLT1) non-transportable inhibitor of L-glutamate and L-aspartate uptake (K_i = 23 μM). Dihydrokainic acid is 130-fold selective over EAAT1 and EAAT3 (K_i > 3 mM). In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT2, EAAT1 and EAAT3, K_i values are 89 μM, > 3 mM and > 3 mM, respectively. Respective K_m values in a FLIPR Membrane Potential (FMP) assay, are 31 μM, > 3 mM and > 3 mM

Technical Data for Dihydrokainic acid

M. Wt	215.25
Formula	C₁₀H₁₇NO₄
Storage	Store at RT
CAS Number	52497-36-6
PubChem ID	107883
InChi Key	JQPDCKOQOOQUSC-OOZYFLPDSA-N
Smiles	O=C(C[C@H]1[C@@H]([C@H](C)C)CN[C@@H]1[C@](O)=O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Dihydrokainic acid

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	5.38	25

Preparing Stock Solutions for Dihydrokainic acid

The following data is based on the product molecular weight 215.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	18.58 mL	92.92 mL	185.83 mL
1.25 mM	3.72 mL	18.58 mL	37.17 mL
2.5 mM	1.86 mL	9.29 mL	18.58 mL
12.5 mM	0.37 mL	1.86 mL	3.72 mL

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References for Dihydrokainic acid

References are publications that support the biological activity of the product.

Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
Kanal The neuronal and epithelial high affinity glutamate transporter, insights into structure and mechanism of transport. J.Biol.Chem. 1994 PMID: 7914198
Munoz Effects of dihydrokainic acid on extracellular amino acids and neuronal excitability in the in vivo rat hippocampus. Neuropharmacology 1987 PMID: 2882438
Arriza Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex. J.Neurosci. 1994 PMID: 7521911