DREADD agonist 21

Name: DREADD agonist 21
Brand: Tocris Bioscience
SKU: 5548
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5548

Citations (15)

COA / SDS

Potent hM3Dq and hM4Di DREADD agonist; blood brain barrier penetrant

Key Product Details

Description:	Potent hM₃D_q and hM₄D_i DREADD agonist; blood brain barrier penetrant
Alternative Names:	Compound 21,C21
Chemical Name:	11-(1-Piperazinyl)-5H-dibenzo[b,e][1,4]diazepine
Purity:	≥98% (HPLC)
Molecular Weight:	278.35

View Full Technical Details for DREADD agonist 21

Technical Data
Citations

Biological Activity for DREADD agonist 21

DREADD agonist 21 is a potent muscarinic DREADD agonist (pEC₅₀ values are 8.91, 8.48 and 7.77 for hM₁D_q, hM₃D_q and hM₄D_i in vitro, respectively). Exhibits > 10-fold higher affinity for hM₁D_q and hM₄D_i compared to wild type receptors. Also displays little to no activity at wild type M₃ receptors. Activates neurons expressing hM₃D_q DREADDS and inhibits activity in neurons expressing hM₄D_i in vivo. Displays excellent brain permeability. Water soluble salt also available, DREADD agonist 21 hydrochloride (Cat. No. 6422).

Technical Data for DREADD agonist 21

M. Wt	278.35
Formula	C₁₇H₁₈N₄
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	56296-18-5
PubChem ID	11818276
InChi Key	JCBYXNSOLUVGTF-UHFFFAOYSA-N
Smiles	C1(N4CCNCC4)=NC(C=CC=C3)=C3NC2=C1C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DREADD agonist 21

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	27.84	100
	ethanol	27.84	100

Preparing Stock Solutions for DREADD agonist 21

The following data is based on the product molecular weight 278.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.59 mL	17.96 mL	35.93 mL
5 mM	0.72 mL	3.59 mL	7.19 mL
10 mM	0.36 mL	1.80 mL	3.59 mL
50 mM	0.07 mL	0.36 mL	0.72 mL

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References for DREADD agonist 21

References are publications that support the biological activity of the product.

Thompson DREADD agonist 21 (C21) is an effective agonist for muscarinic-based DREADDs in vitro and in vivo. ACS Pharmacol.Transl.Sci. 2018 PMID: 30868140
Chen The first structure-activity relationship studies for designer receptors exclusively activated by designer drugs. ACS Chem.Neurosci. 2015 PMID: 25587888