EGCG

Name: EGCG
Brand: Tocris Bioscience
SKU: 4524
Rating: 4 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4524

(3)

Citations (25)

COA / SDS

β-secretase (BACE) inhibitor; inhibits amyloid assembly

Key Product Details

Description:	β-secretase (BACE) inhibitor; inhibits amyloid assembly
Alternative Names:	Epigallocatechin gallate
Chemical Name:	3,4,5-Trihydroxybenzoic acid (2R,3R)-3,4-dihydro-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-2H-1-benzopyran-3-yl ester
Purity:	≥98% (HPLC)
Molecular Weight:	458.37

View Full Technical Details for EGCG

Technical Data
Citations / Reviews (3)

Biological Activity for EGCG

EGCG is a β-secretase (BACE1) inhibitor (IC₅₀ = 1.6 μM); inhibits amyloid assembly. Inhibits the formation of amyloid fibrils by proteins such as Aβ, α-synuclein and huntingtin. Reduces Aβ42-induced cell death in three different cell types. Also inhibits glutamate dehydrogenase. Inhibits cell growth and selectively induces apoptosis in A431 and human pancreatic cancer cells. Also a DNMT1 inhibitor.

Technical Data for EGCG

M. Wt	458.37
Formula	C₂₂H₁₈O₁₁
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	989-51-5
PubChem ID	65064
InChi Key	WMBWREPUVVBILR-WIYYLYMNSA-N
Smiles	O=C(C4=CC(O)=C(O)C(O)=C4)O[C@@H]2CC1=C(O)C=C(O)C=C1O[C@@H]2[C@]3=CC(O)=C(O)C(O)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for EGCG

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	4.58	10
	DMSO	45.84	100

Preparing Stock Solutions for EGCG

The following data is based on the product molecular weight 458.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.91 mL	21.82 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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Reconstitution Calculator

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References for EGCG

References are publications that support the biological activity of the product.

Yang Glioblastoma cells require glutamate dehydrogenase to survive impairments of glucose metabolism or Akt signaling. Cancer Res. 2009 PMID: 19826036
Lee Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids. Mol.Pharmacol. 2005 PMID: 16037419
Shankar EGCG inhibits growth, invasion, angiogenesis and metastastasis of pancreatic cancer. Front.Biosci. 2008 PMID: 17981559
Ahmad Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells. Ach.Biochem.Biophys. 2000 PMID: 10775421
Sinha Comparison of three amyloid assembly inhibitors: the sugar scyllo-inositol, the polyphenol epigallocatechin gallate, and the molecular tweezer CLR01. ACS Chem.Neurosci. 2012 PMID: 22860214
Jeon Green tea catechins as a BACE1 (β-secretase) inhibitor. Bioorg.Med.Chem.Lett. 2003 PMID: 14592472