Filipin III

Name: Filipin III
Brand: Tocris Bioscience
SKU: 6250
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 6250

COA / SDS

Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy

Key Product Details

Description:	Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
Chemical Name:	(3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-Octahydroxy-3-[(1R)-1-hydroxyhexyl]-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one
Purity:	≥90% (HPLC)
Molecular Weight:	654.83

View Full Technical Details for Filipin III

Technical Data
Citations / Reviews (1)

Biological Activity for Filipin III

Key information: filipin III is a fluorescent cholesterol stain; polyene antibiotic.

Used for: fluorescent cholesterol staining. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form.

Application: confocal microscopy.

Properties and Photophysical Data: excitation and emission maxima (λ) are 405 nm and 420-480 nm, respectively.

Technical Data for Filipin III

M. Wt	654.83
Formula	C₃₅H₅₈O₁₁
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	480-49-9
PubChem ID	6433553
InChi Key	IMQSIXYSKPIGPD-ZPXVTHDFSA-N
Smiles	C[C@@H](O1)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@]([C@H](O)CCCCC)([H])C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Filipin III

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.27	5

Preparing Stock Solutions for Filipin III

The following data is based on the product molecular weight 654.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	30.54 mL	152.71 mL	305.42 mL
0.25 mM	6.11 mL	30.54 mL	61.08 mL
0.5 mM	3.05 mL	15.27 mL	30.54 mL
2.5 mM	0.61 mL	3.05 mL	6.11 mL

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References for Filipin III

References are publications that support the biological activity of the product.

Marella Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. J.Biol.Chem. 2002 PMID: 11994310
Pipalia Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells. J.Lipid Res. 2006 PMID: 16288097
Castanho Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J.Biol.Chem. 1992 PMID: 1730589