HTH 01-015

Name: HTH 01-015
Brand: Tocris Bioscience
SKU: 5622

Tocris Bioscience, part of Bio-Techne | Catalog # 5622

Citations (3)

COA / SDS

Potent and selective NUAK1 inhibitor

Key Product Details

Description:	Potent and selective NUAK1 inhibitor
Chemical Name:	5,13-Dihydro-4,5,13-trimethyl-2-[[1-(4-piperidinyl)-1H-pyrazol-4-yl]amino]-6H-naphtho[2,3-e]pyrimido[5,4-b][1,4]diazepin-6-one
Purity:	≥98% (HPLC)
Molecular Weight:	468.55

View Full Technical Details for HTH 01-015

Technical Data
Citations

Biological Activity for HTH 01-015

HTH 01-015 is a potent and selective NUAK1 inhibitor (IC₅₀ = 100 nM). Exhibits no significant inhibition against a panel of 139 kinases, including ten AMPK family members. Inhibits NUAK1-mediated MYPT1 phosphorylation. Also inhibits cell proliferation in U2OS cells in vitro, to a similar extent as NUAK1 knockdown models.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Compound Libraries for HTH 01-015

HTH 01-015 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for HTH 01-015

M. Wt	468.55
Formula	C₂₆H₂₈N₈O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1613724-42-7
PubChem ID	78357766
InChi Key	CHSDJDLAKKAWCI-UHFFFAOYSA-N
Smiles	O=C1N(C)C2=C(N=C(NC3=CN(C4CCNCC4)N=C3)N=C2C)N(C)C5=CC6=CC=CC=C6C=C51

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HTH 01-015

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	46.85	100
	ethanol	46.85	100

Preparing Stock Solutions for HTH 01-015

The following data is based on the product molecular weight 468.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.13 mL	10.67 mL	21.34 mL
5 mM	0.43 mL	2.13 mL	4.27 mL
10 mM	0.21 mL	1.07 mL	2.13 mL
50 mM	0.04 mL	0.21 mL	0.43 mL

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References for HTH 01-015

References are publications that support the biological activity of the product.

Banerjee Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem J. 2014 PMID: 24171924