ISO 1

Name: ISO 1
Brand: Tocris Bioscience
SKU: 4288

Tocris Bioscience, part of Bio-Techne | Catalog # 4288

Citations (11)

COA / SDS

Macrophage migration inhibitory factor (MIF) inhibitor

Key Product Details

Description:	Macrophage migration inhibitory factor (MIF) inhibitor
Chemical Name:	4,5-Dihydro-3-(4-hydroxyphenyl)-5-isoxazoleacetic acid methyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	235.24

View Full Technical Details for ISO 1

Technical Data
Citations

Biological Activity for ISO 1

ISO 1 is a macrophage migration inhibitory factor (MIF) inhibitor (IC₅₀ = 7 μM); inhibits MIF tautomerase activity in vitro and in vivo. Protective against mouse models of streptozotocin-induced diabetes mellitus; blocks anti-inflammatory response following LPS exposure and increases survival. Inhibits airway remodeling in a mouse model of chronic asthma. Cell permeable; orally bioavailable.

Compound Libraries for ISO 1

ISO 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for ISO 1

M. Wt	235.24
Formula	C₁₂H₁₃NO₄
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	478336-92-4
PubChem ID	6098948
InChi Key	CLVQGFPQFNASJH-UHFFFAOYSA-N
Smiles	OC(C=C2)=CC=C2C1=NOC(CC(OC)=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ISO 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	23.52	100
	ethanol	23.52	100

Preparing Stock Solutions for ISO 1

The following data is based on the product molecular weight 235.24.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.25 mL	21.25 mL	42.51 mL
5 mM	0.85 mL	4.25 mL	8.50 mL
10 mM	0.43 mL	2.13 mL	4.25 mL
50 mM	0.09 mL	0.43 mL	0.85 mL

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References for ISO 1

References are publications that support the biological activity of the product.

Lubetsky The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J.Biol.Chem. 2002 PMID: 11997397
Cvetkovic Critical role of macrophage migration inhibitory factor activity in experimental autoimmune diabetes. Endocrinology 2005 PMID: 15790730
Chen ISO-1, a macrophage migration inhibitory factor antagonist, inhibits airway remodeling in a murine model of chronic asthma. Mol.Med. 2010 PMID: 20485865
Al-Abed ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J.Biol.Chem. 2005 PMID: 16115897