ISX 9

Name: ISX 9
Brand: Tocris Bioscience
SKU: 4439

Tocris Bioscience, part of Bio-Techne | Catalog # 4439

Citations (17)

COA / SDS

Neurogenic agent; induces neuronal differentiation of SVZ progenitors and also induces cardiomyogenic differentiation

Key Product Details

Description:	Neurogenic agent; induces neuronal differentiation of SVZ progenitors and also induces cardiomyogenic differentiation
Chemical Name:	N-Cyclopropyl-5-(2-thienyl)-3-isoxazolecarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	234.27

View Full Technical Details for ISX 9

Technical Data
Citations

Biological Activity for ISX 9

ISX 9 is a neurogenic agent. Mediates neuroD reporter gene induction via activation of Ca²⁺ influx; increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. Induces neuronal differentiation in human cortical neuronal cells (HCN), adult mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. ISX 9 is also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro. When used in combination with other small molecules, ISX 9 directs differentiation of pancreatic progenitors towards β cells.

ISX 9 synthesized to Ancillary Material Grade also available.

For more information about how ISX 9 may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.

Compound Libraries for ISX 9

ISX 9 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for ISX 9

M. Wt	234.27
Formula	C₁₁H₁₀N₂O₂S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	832115-62-5
PubChem ID	19582717
InChi Key	SYENTKHGMVKGAQ-UHFFFAOYSA-N
Smiles	O=C(NC2CC2)C1=NOC(C3=CC=CS3)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ISX 9

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	23.43	100
	ethanol	11.71	50

Preparing Stock Solutions for ISX 9

The following data is based on the product molecular weight 234.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.27 mL	21.34 mL	42.69 mL
5 mM	0.85 mL	4.27 mL	8.54 mL
10 mM	0.43 mL	2.13 mL	4.27 mL
50 mM	0.09 mL	0.43 mL	0.85 mL

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References for ISX 9

References are publications that support the biological activity of the product.

Liu Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nat.Commun. 2021 PMID: 34099664
Russell Targeting native adult heart progenitors with cardiogenic small molecules. ACS Chem.Biol. 2012 PMID: 22413910
Dioum A small molecule differentiation inducer increases Ins production by pancreatic β cells. Proc.Natl.Acad.Sci.U.S.A. 2011 PMID: 22143803
Zhang Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression. Differentiation 2011 PMID: 21419563
Schneider Small-molecule activation of neuronal cell fate. Nat.Chem.Biol 2008 PMID: 18552832