JH 295

Name: JH 295
Brand: Tocris Bioscience
SKU: 4322

Tocris Bioscience, part of Bio-Techne | Catalog # 4322

Citations (3)

COA / SDS

Irreversible Nek2 inhibitor

Key Product Details

Description:	Irreversible Nek2 inhibitor
Chemical Name:	N-[(3Z)-3-[(2-Ethyl-4-methyl-1H-imidazol-5-yl)methylene]-2,3-dihydro-2-oxo-1H-indol-5-yl]-2-propynamide
Purity:	≥98% (HPLC)
Molecular Weight:	320.35

View Full Technical Details for JH 295

Technical Data
Citations

Biological Activity for JH 295

JH 295 is an irreversible Nek2 inhibitor (IC₅₀=770 nM). Inhibits Nek2 via alkylation of residue Cys22. Displays 5-fold selectivity for Nek2 over Cdk1. Exhibits no significant effect on Aurora B or Plk1 kinases, bipolar spindle assembly or spindle assembly checkpoint. Cell permeable.

Technical Data for JH 295

M. Wt	320.35
Formula	C₁₈H₁₆N₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1311143-71-1
PubChem ID	53262909
InChi Key	ORKOHXAJFGRZCL-LCYFTJDESA-N
Smiles	O=C2NC1=CC=C(NC(C#C)=O)C=C1/C2=C/C3=C(C)N=C(CC)N3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JH 295

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	32.03	100

Preparing Stock Solutions for JH 295

The following data is based on the product molecular weight 320.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.22 mL
5 mM	0.62 mL	3.12 mL	6.24 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

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References for JH 295

References are publications that support the biological activity of the product.

Henise Irreversible Nek2 kinase inhibitors with cellular activity. J.Med.Chem. 2011 PMID: 21627121