Kaempferol

Name: Kaempferol
Brand: Tocris Bioscience
SKU: 3603
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3603

(1)

Citations (10)

COA / SDS

Proapoptotic; downregulates PLK1 expression and activates mitochondrial Ca2+ uniporter

Key Product Details

Description:	Proapoptotic; downregulates PLK1 expression and activates mitochondrial Ca²⁺ uniporter
Alternative Names:	Kempferol
Chemical Name:	3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	286.24

View Full Technical Details for Kaempferol

Technical Data
Citations / Reviews (1)

Biological Activity for Kaempferol

Activates the mitochondrial Ca²⁺ uniporter (EC₅₀ = 7 μM). Induces caspase-9-mediated apoptosis in cancer cell lines via downregulation of polo-like kinase 1 (PLK1) expression. Exhibits antioxidant activity and attenuates osteoclastic bone reabsorption in vitro. Also blocks EGF-induced histone H3Ser¹⁰ phosphorylation in mouse epidermal JB6 C141 cells. Activates transcriptional coactivator with PDZ-binding motif (TAZ) and increases osteoblast differentiation of mesenchymal stem cells while inhibiting adipocyte differentiation. Naturally occurring flavonoid found in Gingko biloba and red wine. Kaempferol has antiviral activity against Japanese encephalitis virus (JEV) in infected BHK21 (EC₅₀ = 21.5 μM). Kaempferol directly inhibits viral replication and protein expression by binding JEV frameshift site RNA (fsRNA). Kaempferol influences frameshift efficiency in virus-infected cells through interfering with a downstream RNA pseudoknot structure. Kaempferol has also been reported to exhibit activity against herpes simplex virus, influenza viruses (H1N1 and H9N2) and hepatitis B virus under in vitro conditions.

Technical Data for Kaempferol

M. Wt	286.24
Formula	C₁₅H₁₀O₆
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	520-18-3
PubChem ID	5280863
InChi Key	IYRMWMYZSQPJKC-UHFFFAOYSA-N
Smiles	O=C2C1=C(O)C=C(O)C=C1OC(C3=CC=C(O)C=C3)=C2O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Kaempferol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	28.62	100
	ethanol	28.62	100

Preparing Stock Solutions for Kaempferol

The following data is based on the product molecular weight 286.24.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.49 mL	17.47 mL	34.94 mL
5 mM	0.70 mL	3.49 mL	6.99 mL
10 mM	0.35 mL	1.75 mL	3.49 mL
50 mM	0.07 mL	0.35 mL	0.70 mL

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References for Kaempferol

References are publications that support the biological activity of the product.

Byun TAZ is required for the osteogenic and anti-adipogenic activities of kaempferol. Bone 2012 PMID: 22108137
Li In vitro and in vivo anti-hepatitis B virus activities of a plant extract from Geranium carolinianum L. Antiviral Res. 2008 PMID: 18423640
Cho Ribosomal S6 kinase 2 is a key regulator in tumor promoter induced cell transformation. Cancer Res. 2007 PMID: 17804722
Vay Modulation of Ca²⁺ release and Ca²⁺ oscillations in HeLa cells and fibroblasts by mitochondrial Ca²⁺ uniporter stimulation. J.Physiol.
Kang Downregulation of PLK-1 expression in kaempferol-induced apoptosis of MCF-7 cells. Eur.J.Pharmacol. 2009 PMID: 19356725
Suh Kaempferol attenuates 2-deoxy-D-ribose-induced oxidative cell damage in MC3T3-E1 osteoblastic cells. Biol.Pharm.Bull. 2009 PMID: 19336918
Montero Direct activation of the mitochondrial calcium uniporter by natural plant flavonoids. Biochem.J. 2004 PMID: 15324303