KN-62

Tocris Bioscience, part of Bio-Techne | Catalog # 1277

(1)

Citations (151)

COA / SDS

Non-competitive P2X7 antagonist

Key Product Details

Description:	Non-competitive P2X₇ antagonist
Chemical Name:	4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester
Purity:	≥98% (HPLC)
Molecular Weight:	721.84

View Full Technical Details for KN-62

Technical Data
Citations / Reviews (1)

Biological Activity for KN-62

KN-62 is a selective, cell-permeable inhibitor of CaM kinase II (IC₅₀ = 0.9 μM). Binds directly to the calmodulin binding site of the enzyme. Potent non-competitive antagonist at the P2X₇ receptor (IC₅₀ = 15 nM).

Technical Data for KN-62

M. Wt	721.84
Formula	C₃₈H₃₅N₅O₆S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	127191-97-3
PubChem ID	5312126
InChi Key	RJVLFQBBRSMWHX-DHUJRADRSA-N
Smiles	O=S(C1=CC=CC2=C1C=CN=C2)(N(C)[C@H](C(N4CCN(C7=CC=CC=C7)CC4)=O)CC3=CC=C(OS(C5=CC=CC6=C5C=CN=C6)(=O)=O)C=C3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for KN-62

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	72.18	100

Preparing Stock Solutions for KN-62

The following data is based on the product molecular weight 721.84.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.39 mL	6.93 mL	13.85 mL
5 mM	0.28 mL	1.39 mL	2.77 mL
10 mM	0.14 mL	0.69 mL	1.39 mL
50 mM	0.03 mL	0.14 mL	0.28 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for KN-62

References are publications that support the biological activity of the product.

Hidaka and Yokokura Molecular and cellular pharmacology of a calcium/calmodulin-dependent protein kinase II (CaM kinase II) inhibitor, KN-62, and proposal of CaM kinase phosphorylation cascades. Adv.Pharmacol. 1996 PMID: 8783561
Tokumitsu KN-62, 1-[N-O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine, a specific inhibitor of Ca²⁺/calmodulin-dependent protein kinase II. J.Biol.Chem. 1990 PMID: 2155222
Chessell Effects of antagonists at the human recombinant P2X₇ receptor. Br.J.Pharmacol. 1998 PMID: 9720806