L-(-)-threo-3-Hydroxyaspartic acid

Name: L-(-)- threo -3-Hydroxyaspartic acid
Brand: Tocris Bioscience
SKU: 0183

Tocris Bioscience, part of Bio-Techne | Catalog # 0183

Citations (12)

COA / SDS

Transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor

Key Product Details

Description:	Transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor
Alternative Names:	L-threo-β-Hydroxyaspartic acid
Purity:	≥98% (HPLC)
Molecular Weight:	149.1

View Full Technical Details for L-(-)-threo-3-Hydroxyaspartic acid

Technical Data
Citations

Biological Activity for L-(-)-threo-3-Hydroxyaspartic acid

L-(-)-threo-3-Hydroxyaspartic acid is a potent, competitive, transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor. In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT1, EAAT2 and EAAT3, K_i values are 11, 19, and 14 μM, respectively. In a FLIPR Membrane Potential (FMP) assay, K_m values are 3.6, 3.8 and 3.2 μM for human EAAT1, EAAT2 and EAAT3, respectively.

Technical Data for L-(-)-threo-3-Hydroxyaspartic acid

M. Wt	149.1
Formula	C₄H₇NO₅
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	7298-99-9
PubChem ID	443239
InChi Key	YYLQUHNPNCGKJQ-LWMBPPNESA-N
Smiles	[H][C@@]([C@]([H])(O)C(O)=O)(N)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L-(-)-threo-3-Hydroxyaspartic acid

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	1eq. NaOH		100

Preparing Stock Solutions for L-(-)-threo-3-Hydroxyaspartic acid

The following data is based on the product molecular weight 149.1.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.71 mL	33.53 mL	67.07 mL
5 mM	1.34 mL	6.71 mL	13.41 mL
10 mM	0.67 mL	3.35 mL	6.71 mL
50 mM	0.13 mL	0.67 mL	1.34 mL

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References for L-(-)-threo-3-Hydroxyaspartic acid

References are publications that support the biological activity of the product.

Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
McBean and Roberts Neurotoxicity of L-glutamate and DL-threo-3-hydroxyaspartate in the rat striatum. J.Neurochem. 1985 PMID: 2856883
Nakamura (2S,3S,4R)-2-(carboxycyclopropyl)glycine, a potent and competitive inhibitor of both glial and neuronal uptake of glutamate. Neuropharmacology 1993 PMID: 7901789
Johnston Potentiation of L-glutamate and L-aspartate excitation of cat spinal neurones by the stereoisomers of threo-3-hydroxyaspartate. J.Neurochem. 1980 PMID: 7452241