LEI 401

Name: LEI 401
Brand: Tocris Bioscience
SKU: 7407

Tocris Bioscience, part of Bio-Techne | Catalog # 7407

COA / SDS

High affinity N-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) inhibitor

Key Product Details

Description:	High affinity N-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) inhibitor
Chemical Name:	N-(Cyclopropylmethyl)-6-[(3S)-3-hydroxy-1-pyrrolidinyl]-2-[(3S)-3-phenyl-1-piperidinyl]-4-pyrimidinecarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	421.55

View Full Technical Details for LEI 401

Technical Data
Citations

Biological Activity for LEI 401

LEI 401 is a high affinity N-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) inhibitor (K_i = 0.027 and 0.18 μM for human and mouse NAPE-PLD, respectively; IC₅₀ for human enzyme = 0.86 μM). LEI 401 exhibits no significant activity at cannabinoid receptors or other metabolic enzymes of the endocannabinoid system. The product reduces levels of N-acylethanolamines (NAE) in a neuroblastoma cell line in vitro. It also reduces anandamide levels in mouse brain in vivo and impairs fear extinction. LEI 701 is CNS penetrant and cell-permeable.

Licensing Information

Sold with kind permission of Professor van der Stelt

Compound Libraries for LEI 401

LEI 401 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for LEI 401

M. Wt	421.55
Formula	C₂₄H₃₁N₅O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2393840-15-6
PubChem ID	145998143
InChi Key	GFHJYPQZBBHOCC-UXHICEINSA-N
Smiles	O[C@H]1CCN(C2=NC(N3CCC[C@@H](C4=CC=CC=C4)C3)=NC(C(NCC5CC5)=O)=C2)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LEI 401

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	42.16	100
	ethanol	21.1	50

Preparing Stock Solutions for LEI 401

The following data is based on the product molecular weight 421.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.37 mL	11.86 mL	23.72 mL
5 mM	0.47 mL	2.37 mL	4.74 mL
10 mM	0.24 mL	1.19 mL	2.37 mL
50 mM	0.05 mL	0.24 mL	0.47 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for LEI 401

References are publications that support the biological activity of the product.

Mock Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice. Nat.Chem.Biol. 2020 PMID: 32393901