LFM-A13

Name: LFM-A13
Brand: Tocris Bioscience
SKU: 1300

Tocris Bioscience, part of Bio-Techne | Catalog # 1300

Citations (20)

COA / SDS

Potent, selective BTK inhibitor

Key Product Details

Description:	Potent, selective BTK inhibitor
Chemical Name:	2-Cyano-N-(2,5-dibromophenyl)-3-hydroxy-2-butenamide
Purity:	≥98% (HPLC)
Molecular Weight:	360

View Full Technical Details for LFM-A13

Technical Data
Citations

Biological Activity for LFM-A13

LFM-A13 is a potent and selective inhibitor of Bruton's tyrosine kinase (BTK). Inhibits recombinant BTK with an IC₅₀ value of 2.5 μM and has no activity on other protein kinases ( including JAK1, JAK3, HCK, EGFR kinase and insulin receptor kinase) at concentrations of up to 278 μM.

Compound Libraries for LFM-A13

LFM-A13 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for LFM-A13

M. Wt	360
Formula	C₁₁H₈Br₂N₂O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	62004-35-7
PubChem ID	54676905
InChi Key	UVSVTDVJQAJIFG-VURMDHGXSA-N
Smiles	C\C(O)=C(/C#N)C(=O)NC1=C(Br)C=CC(Br)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LFM-A13

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36	100

Preparing Stock Solutions for LFM-A13

The following data is based on the product molecular weight 360.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.78 mL	13.89 mL	27.78 mL
5 mM	0.56 mL	2.78 mL	5.56 mL
10 mM	0.28 mL	1.39 mL	2.78 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

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References for LFM-A13

References are publications that support the biological activity of the product.

Mahajan Rational design and synthesis of a novel anti-leukemic agent targeting Bruton's tyrosine kinase (BTK), LFM-A13 [α-cyano-β-hydroxy-β-methyl-N-(2,5-dibromophenyl)propenamide]. J.Biol.Chem. 1999 PMID: 10092645
Vassilev Bruton's tyrosine kinase as an inhibitor of the Fas/CD95 death-inducing signaling complex. J.Biol.Chem. 1999 PMID: 9880544
Crosby and Pool Interaction of Bruton's tyrosine kinase and protein kinase Cθ in platelets. J.Biol.Chem. 2002 PMID: 11788586