LNT 1

Name: LNT 1
Brand: Tocris Bioscience
SKU: 6510

Tocris Bioscience, part of Bio-Techne | Catalog # 6510

Citations (5)

COA / SDS

Potent flap endonuclease 1 (FEN1) inhibitor; induces DNA damage response

Key Product Details

Description:	Potent flap endonuclease 1 (FEN1) inhibitor; induces DNA damage response
Chemical Name:	1-[(2,3-Dihydro-1,4-benzodioxin-2-yl)methyl]-3-hydroxythieno[3,2-d]pyrimidine-2,4(1H,3H)-dione
Purity:	≥98% (HPLC)
Molecular Weight:	332.33

View Full Technical Details for LNT 1

Technical Data
Citations

Biological Activity for LNT 1

LNT 1 is a potent flap endonuclease 1 (FEN1) inhibitor (IC₅₀ = 46.4 nM for hFEN1-336Δ). Cytotoxic to SW620 colorectal cancer cells in vitro; induces DNA damage response.

Compound Libraries for LNT 1

LNT 1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for LNT 1

M. Wt	332.33
Formula	C₁₅H₁₂N₂O₅S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	824983-91-7
PubChem ID	11652865
InChi Key	MXQGCMQXTPTJJT-UHFFFAOYSA-N
Smiles	ON1C(N(C2=C(C1=O)SC=C2)CC3COC4=CC=CC=C4O3)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LNT 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	16.62	50

Preparing Stock Solutions for LNT 1

The following data is based on the product molecular weight 332.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	6.02 mL	30.09 mL	60.18 mL
2.5 mM	1.20 mL	6.02 mL	12.04 mL
5 mM	0.60 mL	3.01 mL	6.02 mL
25 mM	0.12 mL	0.60 mL	1.20 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for LNT 1

References are publications that support the biological activity of the product.

Ward Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One 2017 PMID: 28628639
Exell Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat.Chem.Bio. 2016 PMID: 27526030