Metformin hydrochloride

Name: Metformin hydrochloride
Brand: Tocris Bioscience
SKU: 2864
Rating: 4 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2864

(2)

Citations (30)

COA / SDS

Activator of LKB1/AMPK; antidiabetic agent

Key Product Details

Description:	Activator of LKB1/AMPK; antidiabetic agent
Chemical Name:	N,N-Dimethylimidodicarbonimidic diamide hydrochloride
Molecular Weight:	165.62

View Full Technical Details for Metformin hydrochloride

Technical Data
Citations / Reviews (2)

Biological Activity for Metformin hydrochloride

Metformin hydrochloride is an antidiabetic agent; lowers plasma glucose levels and improves insulin sensitivity. Inhibits hepatic gluconeogenesis via activation of the LKB1/AMPK pathway. Displays antiproliferative effects in cancer cell lines via inhibition of mitochondrial complex I (MRC-1). Activates the aPKC-CBP pathway in neural precursors to promote neurogenesis. Activates autophagy. Identified by chemoinformatics as targeting human host proteins that interact with SARS-CoV-2. Metformin hydrochloride prevents brain atrophy, and slows the pace of aging across diverse tissues in animal studies.

Compound Libraries for Metformin hydrochloride

Metformin hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library, Tocriscreen FDA-Approved Drugs and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Metformin hydrochloride

M. Wt	165.62
Formula	C₄H₁₁N₅.HCl
Storage	Store at RT
CAS Number	1115-70-4
PubChem ID	14219
InChi Key	OETHQSJEHLVLGH-UHFFFAOYSA-N
Smiles	NC(NC(N(C)C)=N)=N.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Metformin hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	16.56	100
	DMSO	3.31	20 with gentle warming

Preparing Stock Solutions for Metformin hydrochloride

The following data is based on the product molecular weight 165.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.04 mL	30.19 mL	60.38 mL
5 mM	1.21 mL	6.04 mL	12.08 mL
10 mM	0.60 mL	3.02 mL	6.04 mL
50 mM	0.12 mL	0.60 mL	1.21 mL

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References for Metformin hydrochloride

References are publications that support the biological activity of the product.

Yang Metformin decelerates aging clock in male monkeys. Cell 2024 PMID: 39270656
Wheaton MetfF inhibits mitochondrial complex I of cancer cells to reduce tumorigenesis. Elife 2014 PMID: 24843020
Gordon A SARS-CoV-2-human protein-protein interaction map reveals drug targets and potential drug-repurposing. Nature 2020 PMID: 32353859
Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
Wang MetF. activates an atypical PKC-CBP pathway to promote neurogenesis and enhance spatial memory formation. Cell Stem Cell 2012 PMID: 22770240
Hirsch MetF. inhibits the inflammatory response associated with cellular transformation and cancer stem cell growth. Proc.Natl.Acad.Sci.USA 2013 PMID: 23277563
Towler and Hardie AMP-activated protein kinase in metabolic control and Ins signalling. Circ.Res. 2007 PMID: 17307971
Shaw The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of MetF.. Science 2005 PMID: 16308421