ML 67-33

Name: ML 67-33
Brand: Tocris Bioscience
SKU: 6886

Tocris Bioscience, part of Bio-Techne | Catalog # 6886

Citations (2)

COA / SDS

K2P2.1 (TREK-1), K2P10.1 (TREK-2) and K2P4.1 (TRAAK) channel activator

Key Product Details

Description:	K_2P2.1 (TREK-1), K_2P10.1 (TREK-2) and K_2P4.1 (TRAAK) channel activator
Chemical Name:	2,7-Dichloro-9,10-dihydro-9,9-dimethyl-10-[2-(2H-tetrazol-5-yl)ethyl]acridine
Purity:	≥98% (HPLC)
Molecular Weight:	374.27

View Full Technical Details for ML 67-33

Technical Data
Citations

Biological Activity for ML 67-33

ML 67-33 is a K_2P potassium channel activator (EC₅₀ values are 21.8 - 29.4 μM, 30.2 μM and 27.3 μM for K_2P2.1 (TREK-1), K_2P10.1 (TREK-2) and K_2P4.1 (TRAAK) respectively, expressed in xenopus oocytes). Increases channel currents by activating core gating apparatus of channels. In a mouse migraine model, ML 67-33 improves pain symptoms via activation of TREK 1/2 currents in trigeminal ganglion sensory neurons.

Compound Libraries for ML 67-33

ML 67-33 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 67-33

M. Wt	374.27
Formula	C₁₈H₁₇Cl₂N₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1443290-89-8
PubChem ID	86272454
InChi Key	GQPMJOXQJUGJPG-UHFFFAOYSA-N
Smiles	CC1(C2=CC(Cl)=CC=C2N(C3=CC=C(C=C13)Cl)CCC4=NN=NN4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 67-33

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	37.43	100

Preparing Stock Solutions for ML 67-33

The following data is based on the product molecular weight 374.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.67 mL	13.36 mL	26.72 mL
5 mM	0.53 mL	2.67 mL	5.34 mL
10 mM	0.27 mL	1.34 mL	2.67 mL
50 mM	0.05 mL	0.27 mL	0.53 mL

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References for ML 67-33

References are publications that support the biological activity of the product.

Pope Protein and chemical determinants of BL-1249 action and selectivity for K_2P channels. ACS Chem.Neurosci. 2018 PMID: 30089357
Prado TREK channel activation suppresses migraine pain phenotype. iScience 2021 PMID: 34458705
Bagriantsev A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K_2P channels. ACS Chem.Biol. 2013 PMID: 23738709