MPD 2

Name: MPD 2
Brand: Tocris Bioscience
SKU: 8802

Tocris Bioscience, part of Bio-Techne | Catalog # 8802

COA / SDS

SARS-CoV-2 Mpro Degrader (PROTAC®)

Key Product Details

Description:	SARS-CoV-2 M^pro Degrader (PROTAC^®)
Chemical Name:	3-((6-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)hexyl)oxy)benzyl ((2S,3R)-3-(tert-butoxy)-1-(((S)-3-cyclohexyl-1-oxo-1-(((S)-1-oxo-3-((S)-2-oxopyrrolidin-3-yl)propan-2-yl)amino)propan-2-yl)amino)-1-oxobutan-2-yl)carbamate
Molecular Weight:	973.13

View Full Technical Details for MPD 2

Technical Data
Citations

Biological Activity for MPD 2

MPD 2 is a SARS-CoV-2 M^pro Degrader (PROTAC^®) (DC₅₀ = 296 nM in 293T cells). M^pro degradation is time-dependent and CRBN-mediated. Antiviral in A549-ACE2 cells infected with several SARS-CoV-2 strains (EC₅₀ = 492 nM at delta variant) and against a nirmatrelvir-resistant mutant of SARS-CoV-2, NSP5 E166A (EC₅₀ = 460 nM).

Scientific Data Examples for MPD 2

	Application of MPD 2. Western blot data showing dose responsive degradation with MPD 2 on transiently expressed SARS-CoV-2 Main Protease in HEK293 cells. 1st Ab: HRP Anti-V5 tag antibody [E10/V4RR] (ab173837) (1:2000), 2nd Ab: Anti-Mouse HRP (1:5000). Loading control: 1st Ab: Anti-alpha - Tubulin antibody [DM1A] - (1:10000) 2nd Ab: Anti-mouse HRP (1:5000). Data provided by Brönstrup Lab at the Helmholtz Centre for Infection Research.

Technical Data for MPD 2

M. Wt	973.13
Formula	C₅₁H₆₈N₆O₁₃
Storage	Store at -20°C
PubChem ID	171663340
InChi Key	ZKSDAFLRLHGTNH-DTKLLKMQSA-N
Smiles	O=C1[C@@H](CCN1)C[C@H](NC([C@@H](NC([C@@H](NC(OCC2=CC(OCCCCCCOC3=C4C(C(N(C4=O)C5CCC(NC5=O)=O)=O)=CC=C3)=CC=C2)=O)[C@@H](C)OC(C)(C)C)=O)CC6CCCCC6)=O)C([H])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MPD 2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	97.31	100

Preparing Stock Solutions for MPD 2

The following data is based on the product molecular weight 973.13.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.03 mL	5.14 mL	10.28 mL
5 mM	0.21 mL	1.03 mL	2.06 mL
10 mM	0.10 mL	0.51 mL	1.03 mL
50 mM	0.02 mL	0.10 mL	0.21 mL

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References for MPD 2

References are publications that support the biological activity of the product.

Alugubelli Discovery of first-in-class PROTAC Degraders of SARS-CoV-2 main protease. J.Med.Chem. 2024 PMID: 38608245