N-Acetylcysteine

Name: N -Acetylcysteine
Brand: Tocris Bioscience
SKU: 7874

Tocris Bioscience, part of Bio-Techne | Catalog # 7874

Citations (26)

COA / SDS

Glutathione (GSH) precursor; maintains stem cell function in culture.

Key Product Details

Description:	Glutathione (GSH) precursor; maintains stem cell function in culture.
Chemical Name:	(2R)-2-Acetamido-3-sulfanylpropanoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	163.2

View Full Technical Details for N-Acetylcysteine

Technical Data
Citations

Biological Activity for N-Acetylcysteine

N-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.

Technical Data for N-Acetylcysteine

M. Wt	163.2
Formula	C₅H₉NO₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	616-91-1
PubChem ID	12035
InChi Key	PWKSKIMOESPYIA-BYPYZUCNSA-N
Smiles	[C@@H](NC(C)=O)(C(O)=O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for N-Acetylcysteine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	8.16	50
	water	8.16	50

Preparing Stock Solutions for N-Acetylcysteine

The following data is based on the product molecular weight 163.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	12.25 mL	61.27 mL	122.55 mL
2.5 mM	2.45 mL	12.25 mL	24.51 mL
5 mM	1.23 mL	6.13 mL	12.25 mL
25 mM	0.25 mL	1.23 mL	2.45 mL

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Reconstitution Calculator

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References for N-Acetylcysteine

References are publications that support the biological activity of the product.

Geiler N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus. Biochem.Pharmacol. 2009 PMID: 19732754
Moiseeva Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature 2023 PMID: 36544018
Broutier Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat.Protoc. 2016 PMID: 27560176
Balboa Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. Nat.Biotechnol. 2022 PMID: 35241836
Cattaneo Tumor organoid-T-cell coculture systems. Nat.Protoc. 2019 PMID: 31853056