Necrostatin-1

Name: Necrostatin-1
Brand: Tocris Bioscience
SKU: 2324

Tocris Bioscience, part of Bio-Techne | Catalog # 2324

(2)

Citations (47)

COA / SDS

RIP1 kinase inhibitor; inhibits necroptosis

Key Product Details

Chemical Name:	5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity:	≥98%
Molecular Weight:	259.33 kDa

View Full Technical Details for Necrostatin-1

Technical Data
Citations / Reviews (2)

Biological Activity for Necrostatin-1

Necrostatin-1 is an ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC₅₀ = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC₅₀ = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Technical Data for Necrostatin-1

M. Wt	259.33 kDa
Formula	C₁₃H₁₃N₃OS
Storage	Store at RT
Purity	≥98%
CAS Number	4311-88-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Necrostatin-1

Solubility

Soluble Soluble to 30 mM in DMSO

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Necrostatin-1

References are publications that support the biological activity of the product.

Zhao C, Pavicic P, Datta S, Sun D, Novotny M, Hamilton T Cellular stress amplifies TLR3/4-induced CXCL1/2 gene transcription in mononuclear phagocytes via RIPK1. J Immunol, 2014;193(2):879-88. 2014 PMID: 24920846
HJ Kim, KE Hwang, DS Park, SH Oh, HY Jun, KH Yoon, ET Jeong, HR Kim, YS Kim Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells J Transl Med, 2017;15(1):123. 2017 PMID: 28569199
B Thibault, L Genre, A Le Naour, C Broca, E Mery, G Vuagniaux, JP Delord, N Wiedemann, B Couderc DEBIO 1143, an IAP inhibitor, reverses carboplatin resistance in ovarian cancer cells and triggers apoptotic or necroptotic cell death Sci Rep, 2018;8(1):17862. 2018 PMID: 30552344
M Lötzerich, PS Roulin, K Boucke, R Witte, O Georgiev, UF Greber Rhinovirus 3C protease suppresses apoptosis and triggers caspase-independent cell death Cell Death Dis, 2018;9(3):272. 2018 PMID: 29449668
SW Alvarez, VO Sviderskiy, EM Terzi, T Papagianna, AL Moreira, S Adams, DM Sabatini, K Birsoy, R Possemato NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis Nature, 2017;551(7682):639-643. 2017 PMID: 29168506
Degterev et al. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat.Chem.Biol., 2005;1:112 PMID: 16408008
Degterev et al. Identification of RIP1 kinase as a specific cellular target of necroSTAT. Nat.Chem.Biol., 2008;4:313 PMID: 18408713