Ned K

Name: Ned K
Brand: Tocris Bioscience
SKU: 5976

Tocris Bioscience, part of Bio-Techne | Catalog # 5976

Citations (1)

COA / SDS

Prevents Ca2+ oscillations in cardiomyocytes during ischemia/reperfusion; NAADP signaling inhibitor

Key Product Details

Description:	Prevents Ca²⁺ oscillations in cardiomyocytes during ischemia/reperfusion; NAADP signaling inhibitor
Chemical Name:	(1R,3S)-1-[3-[[4-(2-Cyanophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	521.61

View Full Technical Details for Ned K

Technical Data
Citations

Biological Activity for Ned K

Ned K is a NAADP inhibitor. Prevents Ca²⁺ oscillations in rat cardiomyocytes in vitro during simulated ischemia and reperfusion, but exhibits no significant effect on Ca²⁺ sparks. Significantly reduces infarct size in rat cardiac ischemia/reperfusion model in vivo.

Technical Data for Ned K

M. Wt	521.61
Formula	C₃₁H₃₁N₅O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2250019-90-8
PubChem ID	127021041
InChi Key	LMTWTJMBOFXQTH-LITSAYRRSA-N
Smiles	O=C(O)[C@H]1N[C@H](C2=CC=C(OC)C(CN3CCN(C4=CC=CC=C4C#N)CC3)=C2)C5=C(C(C=CC=C6)=C6N5)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ned K

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	26.08	50

Preparing Stock Solutions for Ned K

The following data is based on the product molecular weight 521.61.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.83 mL	19.17 mL	38.34 mL
2.5 mM	0.77 mL	3.83 mL	7.67 mL
5 mM	0.38 mL	1.92 mL	3.83 mL
25 mM	0.08 mL	0.38 mL	0.77 mL

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References for Ned K

References are publications that support the biological activity of the product.

Davidson Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore. Cardiovasc.Res. 2015 PMID: 26395965