NF 449

Tocris Bioscience, part of Bio-Techne | Catalog # 1391

COA / SDS

Highly selective P2X1 antagonist

Key Product Details

Description:	Highly selective P2X₁ antagonist
Chemical Name:	4,4',4'',4'''-[Carbonylbis(imino-5,1,3-benzenetriyl-bis(carbonylimino))]tetrakis-1,3-benzenedisulfonic acid, octasodium salt
Purity:	≥90% (HPLC)
Molecular Weight:	1505.06

View Full Technical Details for NF 449

Technical Data
Citations

Biological Activity for NF 449

NF 449 is a potent purinergic receptor antagonist that displays high selectivity for P2X₁ (IC₅₀ values are 0.28, 0.69, 120, 1820, 47000 and > 300000 nM for rP2X₁, rP2X₁₊₅, rP2X₂₊₃, rP2X₃, rP2X₂ and P2X₄ receptors respectively). Provides antithrombotic protection in vivo. Also acts as a G_sα-selective antagonist. Also inhibits DNA-binding activity of HMGA2 (IC₅₀ = 0.43 μM).

This product is supplied with a high degree of hydration and some residual NaCl, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content and the maximum solubility threshold to use in dilution calculations.

Technical Data for NF 449

M. Wt	1505.06
Formula	C₄₁H₂₄N₆Na₈O₂₉S₈
Storage	Store at RT
Purity	≥90% (HPLC)
CAS Number	627034-85-9
PubChem ID	91895233
InChi Key	PHCBPKWKKHYRSA-UHFFFAOYSA-F
Smiles	[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]S(=O)(=O)C1=CC=C(NC(=O)C2=CC(=CC(NC(=O)NC3=CC(=CC(=C3)C(=O)NC3=CC=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)C(=O)NC3=CC=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)=C2)C(=O)NC2=CC=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(=C1)S([O-])(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NF 449

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	25

Preparing Stock Solutions for NF 449

The following data is based on the product molecular weight 1505.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.17 mM	3.91 mL	19.54 mL	39.08 mL
0.85 mM	0.78 mL	3.91 mL	7.82 mL
1.7 mM	0.39 mL	1.95 mL	3.91 mL
8.5 mM	0.08 mL	0.39 mL	0.78 mL

Molarity Calculator

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Reconstitution Calculator

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References for NF 449

References are publications that support the biological activity of the product.

Su Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays. Sci.Rep. 2020 PMID: 33139812
Fleming Chemical modulators of autophagy as biological probes and potential therapeutics. Nat.Chem.Biol. 2011 PMID: 21164513
Hohenegger G_sα-selective G protein antagonists. Proc.Natl.Acad.Sci.U.S.A. 1998 PMID: 9419378
Rettinger Profiling at recombinant homomeric and heteromeric rat P2X receptors identifies the suramin analogue NF449 as a highly potent P2X₁ receptor antagonist. Neuropharmacology. 2005 PMID: 15721178
Hechler Inhibition of platelet functions and thrombosis through selective or non-selective inhibition of the platelet P2 receptors with increasing doses of NF449 [4,4',4",4'"-(carbonylbis(imino-5,1,3-benzenetriylbis-(carbonylimino)))tetrakis-benze J.Pharmacol.Exp.Ther. 2005 PMID: 15792995