Niclosamide

Name: Niclosamide
Brand: Tocris Bioscience
SKU: 4079

Tocris Bioscience, part of Bio-Techne | Catalog # 4079

Citations (9)

COA / SDS

STAT3 inhibitor; also inhibits mTORC1 signaling

Key Product Details

Description:	STAT3 inhibitor; also inhibits mTORC1 signaling
Chemical Name:	5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxybenzamide
Purity:	≥99% (HPLC)
Molecular Weight:	325.99

View Full Technical Details for Niclosamide

Technical Data
Citations

Biological Activity for Niclosamide

Niclosamide is an inhibitor of the STAT3 signaling pathway; inhibits the activation, nuclear translocation and transactivation of STAT3. Niclosamide displays selectivity for STAT3 over STAT1, STAT5, JAK1, JAK2 and Src kinases. It inhibits the transcription of STAT3 target genes and induces cell growth inhibition, apoptosis and cell cycle arrest of cancer cells with constitutively active STAT3. It also reversibly inhibits mTORC1 signaling and stimulates autophagy in vitro; displays antineoplastic effects in acute myelogenous leukemia (AML) stem cells. In addition, niclosamide inhibits SARS-CoV-2 replication in vitro (IC₅₀ = 0.34 μM in Vero E6 plaque assay) through suppression of TMEM16F activity.

Technical Data for Niclosamide

M. Wt	325.99
Formula	C₁₃H₈Cl₂N₂O₄
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	50-65-7
PubChem ID	4477
InChi Key	RJMUSRYZPJIFPJ-UHFFFAOYSA-N
Smiles	O=C(NC2=C(Cl)C=C([N+]([O-])=O)C=C2)C1=C(O)C=CC(Cl)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Niclosamide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	8.15	25
	DMSO	3.26	10

Preparing Stock Solutions for Niclosamide

The following data is based on the product molecular weight 325.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	12.27 mL	61.35 mL	122.70 mL
1.25 mM	2.45 mL	12.27 mL	24.54 mL
2.5 mM	1.23 mL	6.14 mL	12.27 mL
12.5 mM	0.25 mL	1.23 mL	2.45 mL

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References for Niclosamide

References are publications that support the biological activity of the product.

Braga Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia. Nature 2021 PMID: 33827113
Jin Antineoplastic mechanisms of niclosamide in acute myelogenous leukemia stem cells: inactivation of the NF-κB pathway and generation of reactive oxygen species. Cancer Res. 2010 PMID: 20215516
Ren Identification of niclosamide as a new small-molecule inhibitor of the STAT3 signaling pathway. ACS Med.Chem.Lett. 2010 PMID: 24900231
Balgi Screen for chemical modulators of autophagy reveals novel therapeutic inhibitors of mTORC1 signaling. PLoS One 2009 PMID: 19771169