Nocodazole

Name: Nocodazole
Brand: Tocris Bioscience
SKU: 1228
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1228

(1)

Citations (113)

COA / SDS

Microtubule inhibitor

Key Product Details

Description:	Microtubule inhibitor
Chemical Name:	[5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]carbonic acid, methyl ester
Purity:	≥95% (HPLC)
Molecular Weight:	301.32

View Full Technical Details for Nocodazole

Technical Data
Citations / Reviews (1)

Biological Activity for Nocodazole

Nocodazole is a microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.

Technical Data for Nocodazole

M. Wt	301.32
Formula	C₁₄H₁₁N₃O₃S
Storage	Store at RT
Purity	≥95% (HPLC)
CAS Number	31430-18-9
PubChem ID	4122
InChi Key	KYRVNWMVYQXFEU-UHFFFAOYSA-N
Smiles	COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nocodazole

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	15.07	50

Preparing Stock Solutions for Nocodazole

The following data is based on the product molecular weight 301.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	6.64 mL	33.19 mL	66.37 mL
2.5 mM	1.33 mL	6.64 mL	13.27 mL
5 mM	0.66 mL	3.32 mL	6.64 mL
25 mM	0.13 mL	0.66 mL	1.33 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Nocodazole

References are publications that support the biological activity of the product.

Lin Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 2014 PMID: 25497837
Webb Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 2004 PMID: 15325591
Mizushima Methods in mammalian autophagy research. Cell 2010 PMID: 20144757
Vasquez Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 1997 PMID: 9201709