Orlistat

Name: Orlistat
Brand: Tocris Bioscience
SKU: 3540

Tocris Bioscience, part of Bio-Techne | Catalog # 3540

Citations (11)

COA / SDS

Pancreatic, gastric and carboxylester lipase inhibitor; antiobesity and antihypercholesterolemic activity

Key Product Details

Description:	Pancreatic, gastric and carboxylester lipase inhibitor; antiobesity and antihypercholesterolemic activity
Chemical Name:	N-Formyl-L-leucine (1S)-1-[[(2S,3S)-3-hexyl-4-oxo-2-oxetanyl]methyl]dodecyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	495.73

View Full Technical Details for Orlistat

Technical Data
Citations

Biological Activity for Orlistat

Orlistat is a hypolipemic pancreatic, gastric and carboxylester lipase inhibitor. Exhibits no activity at phospholipase A₂, liver esterase, trypsin and chymotrypsin. Inhibits the thioesterase domain of fatty acid synthase, leading to cell cycle arrest at the G₁/S boundary in vitro. Prevents the absorption of approximately one third of fat from food and exhibits progastrokinetic, antiobesity and antihypercholesterolemic activity in vivo. Orlistat inhibits neutral lipid lipase LIPE and dose-dependently reduces α-synuclein-positive cytoplasmic inclusions in α-synuclein-expressing neuroblastoma cell lines.

Technical Data for Orlistat

M. Wt	495.73
Formula	C₂₉H₅₃NO₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	96829-58-2
PubChem ID	3034010
InChi Key	AHLBNYSZXLDEJQ-FWEHEUNISA-N
Smiles	O=C1[C@@H](CCCCCC)[C@H](C[C@@H](O[C@]([C@H](CC(C)C)NC([H])=O)=O)CCCCCCCCCCC)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Orlistat

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	49.57	100
	ethanol	49.57	100

Preparing Stock Solutions for Orlistat

The following data is based on the product molecular weight 495.73.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.02 mL	10.09 mL	20.17 mL
5 mM	0.40 mL	2.02 mL	4.03 mL
10 mM	0.20 mL	1.01 mL	2.02 mL
50 mM	0.04 mL	0.20 mL	0.40 mL

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References for Orlistat

References are publications that support the biological activity of the product.

Fanning Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy. NPJ Parkinsons Dis. 2022 PMID: 35680956
Kridel Orlistat is a novel inhibitor of fatty acid synthase with antitumor activity. Cancer Res. 2004 PMID: 15026345
Enc Orlistat accelerates gastric emptying and attenuates GIP release in healthy subjects. Am.J.Physiol.Gastrointest.Liver Physiol. 2008 PMID: 19109408
Hadvary The lipase inhibitor tetrahydrolipstatin binds covalently to the putative active site serine of pancreatic lipase. J.Biol.Chem. 1991 PMID: 1899234