Ouabain

Name: Ouabain
Brand: Tocris Bioscience
SKU: 1076

Tocris Bioscience, part of Bio-Techne | Catalog # 1076

Citations (47)

COA / SDS

Na+/K+ ATPase inhibitor

Key Product Details

Description:	Na⁺/K⁺ ATPase inhibitor
Chemical Name:	3-[(6-Deoxy-α-L-mannopyranosyl)oxy]-1,5,11α,14,19-pentahydoxycard-20(22)-enolide
Purity:	≥95% (HPLC)
Molecular Weight:	584.66

View Full Technical Details for Ouabain

Technical Data
Citations

Biological Activity for Ouabain

Ouabain is a selective Na⁺, K⁺-ATPase inhibitor. Cardiotonic agent. Also exhibits anti-MERS-CoV activity in Vero cells in vitro (IC₅₀ = 0.08 μM).

Technical Data for Ouabain

M. Wt	584.66
Formula	C₂₉H₄₄O₁₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	630-60-4
PubChem ID	439501
InChi Key	LPMXVESGRSUGHW-MJARETDLSA-N
Smiles	O[C@@]12[C@@]3([H])[C@]([C@]([C@H](O)C[C@H](O[C@H]4C(O)C(O)[C@@H](O)[C@H](C)O4)C5)(CO)[C@]5(O)CC3)([H])[C@H](O)C[C@]1(C)[C@@H](C(CO6)=CC6=O)CC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ouabain

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water		10
	DMSO	58.47	100

Preparing Stock Solutions for Ouabain

The following data is based on the product molecular weight 584.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.71 mL	8.55 mL	17.10 mL
5 mM	0.34 mL	1.71 mL	3.42 mL
10 mM	0.17 mL	0.86 mL	1.71 mL
50 mM	0.03 mL	0.17 mL	0.34 mL

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Reconstitution Calculator

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References for Ouabain

References are publications that support the biological activity of the product.

Ko Screening of FDA-approved drugs using a MERS-CoV clinical isolate from South Korea identifies potential therapeutic options for COVID-19. BioRxiv - Paper not yet peer reviewed
Hosoi Isoform-specific up-regulation by ouabain of Na⁺, K⁺-ATPase in cultured rat astrocytes. J.Neurochem. 1997 PMID: 9349566
Marjorie Ouabain improves cardiac function in vivo in rats with heart failure after chronic but not acute treatment. Naunyn Schmiedebergs Arch.Pharmacol. 1997 PMID: 9272726
Croyle Extensive random mutagenesis analysis of the Na/K⁺ ATPase alpha subunit identifies known and previously unidentified amino acid residues that alter ouabain sensitivity-implications for ouabain binding. Eur.J.Biochem. 1997 PMID: 9346307