PA Nic

Name: PA Nic
Brand: Tocris Bioscience
SKU: 6700

Tocris Bioscience, part of Bio-Techne | Catalog # 6700

Citations (2)

COA / SDS

Caged nicotine; photoactivated by one-photon and two-photon excitation

Key Product Details

Description:	Caged nicotine; photoactivated by one-photon and two-photon excitation
Chemical Name:	(1_S,2_S)-1-((7-(Bis(carboxymethyl)amino)-2-oxo-2H-chromen-4-yl)methyl)-1-methyl-2-(pyridin-3-yl)pyrrolidin-1-ium ditrifluoroacetate
Purity:	≥98% (HPLC)
Molecular Weight:	679.52

View Full Technical Details for PA Nic

Technical Data
Citations

Biological Activity for PA Nic

PA Nic is a coumarin-caged nicotine. Releases nicotine when exposed to 390 ± 10 nm wavelength light (λmax = 404 nm). Suitable for two-photon uncaging at <900 nm (Maximum two-photon cross-section at 810 nm). Extinction coefficient (ε) 17,400 M^-1cm^-1. Evokes nicotinic currents in mouse brain slices under one and two-photon activation conditions.

Technical Data for PA Nic

M. Wt	679.52
Formula	C₂₄H₂₆N₃O₆.C₂F₃O₂.C₂HF₃O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
Smiles	C[N@@+]1(CC2=CC(OC3=C2C=CC(N(CC(O)=O)CC(O)=O)=C3)=O)CCC[C@H]1C4=CC=CN=C4.OC(C(F)(F)F)=O.[O-]C(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PA Nic

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	16.99	25
	DMSO	16.99	25

Preparing Stock Solutions for PA Nic

The following data is based on the product molecular weight 679.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	5.89 mL	29.43 mL	58.87 mL
1.25 mM	1.18 mL	5.89 mL	11.77 mL
2.5 mM	0.59 mL	2.94 mL	5.89 mL
12.5 mM	0.12 mL	0.59 mL	1.18 mL

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References for PA Nic

References are publications that support the biological activity of the product.

Yan Nicotinic cholinergic receptors in VTA glutamate neurons modulate excitatory transmission. Cell Rep. 2018 PMID: 29791835
Arvin Probing nicotinic acetylcholine receptor function in mouse brain slices via laser flash photolysis of photoactivatable nicotine. J.Vis.Exp. 2019 PMID: 30735191
Banala Photoactivatable drugs for nicotinic optopharmacology. Nat.Methods 2018 PMID: 29578537