Panobinostat

Name: Panobinostat
Brand: Tocris Bioscience
SKU: 7629

Tocris Bioscience, part of Bio-Techne | Catalog # 7629

Citations (3)

COA / SDS

Pan-histone deacetylase inhibitor

Key Product Details

Description:	Pan-histone deacetylase inhibitor
Chemical Name:	(2E)-N-Hydroxy-3-[4-[[[2-(2-methyl-1H-indol-3-yl)ethyl]amino]methyl]phenyl]-2-propenamide
Purity:	≥98% (HPLC)
Molecular Weight:	349.43

View Full Technical Details for Panobinostat

Technical Data
Citations

Biological Activity for Panobinostat

Panobinostat is a potent pan-histone deacetylase (HDAC) inhibitor (IC₅₀ values = 2.1 - 531 nM). Panobinostat induces histone H3 and H4 acetylation and potently inhibits cell proliferation and cell viability in HH, BT474 and HCT116 cells (IC₅₀ are 1.8, 2.6 and 7.1 nM, respectively) in vitro. It leads to significant tumor regression of up to 94% in an HH CTCL mouse xenograft model. Panobinostat inhibits the DNA binding activity of STAT5 in leukemia cell lines and acts synergistically with 17-AAG (Cat. No. 1515) to induce apoptosis. The compound shows potent antimyeloma activity, including in drug-resistant cell lines. Panobinostat also induces a significant increase in cell-associated unspliced (CA-US) HIV-1 RNA from CD4⁺ T cells and leads to activation of transcription of proviruses. Panobinostat simultaneously suppresses the expression of angiotensin-converting enzyme 2 (ACE2) and ABO in epithelial cell lines. Antimalarial and orally bioavailable.

Technical Data for Panobinostat

M. Wt	349.43
Formula	C₂₁H₂₃N₃O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	404950-80-7
PubChem ID	6918837
InChi Key	FPOHNWQLNRZRFC-ZHACJKMWSA-N
Smiles	ONC(/C=C/C(C=C1)=CC=C1CNCCC2=C(C)NC3=CC=CC=C23)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Panobinostat

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	34.94	100

Preparing Stock Solutions for Panobinostat

The following data is based on the product molecular weight 349.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.86 mL	14.31 mL	28.62 mL
5 mM	0.57 mL	2.86 mL	5.72 mL
10 mM	0.29 mL	1.43 mL	2.86 mL
50 mM	0.06 mL	0.29 mL	0.57 mL

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References for Panobinostat

References are publications that support the biological activity of the product.

Takahashi Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19. Sci.Rep. 2021 PMID: 33564039
Barton Broad activation of latent HIV-1 in vivo. Nat.Commun. 2016 PMID: 27605062
Maiso The histone deacetylase inhibitor LBH589 is a potent antimyeloma agent that overcomes drug resistance. Cancer Res. 2006 PMID: 16740717
George Combination of the histone deacetylase inhibitor LBH589 and the hsp90 inhibitor 17-AAG is highly active against human CML-BC cells and AML cells with activating mutation of FLT-3. Blood 2005 PMID: 15514006
Atadja Development of the pan-DAC inhibitor panobinostat (LBH589): successes and challenges. Cancer Lett. 2009 PMID: 19344997