Phorbol 12,13-dibutyrate

Name: Phorbol 12,13-dibutyrate
Brand: Tocris Bioscience
SKU: 4153
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4153

(1)

Citations (19)

COA / SDS

Protein kinase C activator

Key Product Details

Description:	Protein kinase C activator
Alternative Names:	PDBu
Chemical Name:	(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity:	≥97% (HPLC)
Molecular Weight:	504.61

View Full Technical Details for Phorbol 12,13-dibutyrate

Technical Data
Citations / Reviews (1)

Biological Activity for Phorbol 12,13-dibutyrate

Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca²⁺ concentration. Also inhibits the activity of Na⁺,K⁺ ATPase in OK cells.

For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for Phorbol 12,13-dibutyrate

M. Wt	504.61
Formula	C₂₈H₄₀O₈
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	37558-16-0
PubChem ID	37783
InChi Key	BQJRUJTZSGYBEZ-YVQNUNKESA-N
Smiles	OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Phorbol 12,13-dibutyrate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.23	50

Preparing Stock Solutions for Phorbol 12,13-dibutyrate

The following data is based on the product molecular weight 504.61.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.96 mL	19.82 mL	39.63 mL
2.5 mM	0.79 mL	3.96 mL	7.93 mL
5 mM	0.40 mL	1.98 mL	3.96 mL
25 mM	0.08 mL	0.40 mL	0.79 mL

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References for Phorbol 12,13-dibutyrate

References are publications that support the biological activity of the product.

Hori Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 1999 PMID: 10460232
Sakai Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 2010 PMID: 21187674
Middleton Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 1993 PMID: 8393456