(+)-JQ1 maleimide

Name: (+)-JQ1 maleimide
Brand: Tocris Bioscience
SKU: 7576

Tocris Bioscience, part of Bio-Techne | Catalog # 7576

COA / SDS

JQ1-maleimide functionalized probe; induces proteasomal degradation of BRD4

Key Product Details

Description:	JQ1-maleimide functionalized probe; induces proteasomal degradation of BRD4
Chemical Name:	(S)-N-(2-(2-(2-(2-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)ethoxy)ethoxy)ethyl)-6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanamide
Purity:	≥95% (HPLC)
Molecular Weight:	724.27

View Full Technical Details for (+)-JQ1 maleimide

Technical Data
Citations

Biological Activity for (+)-JQ1 maleimide

(+)-JQ1 maleimide is a probe that comprises a cysteine-reactive maleimide connected to the BRD4 ligand, JQ1, via a PEG2 linker. It can be used in the COFFEE method (COvalent Functionalization Followed by E3 Electroporation) in which (+)-JQ1 maleimide is covalently attached to VHL followed by electroporation into live cells. The E3 ligase forms a complex with BRD4 intracellularly and induces its degradation.

Technical Data for (+)-JQ1 maleimide

M. Wt	724.27
Formula	C₃₅H₄₂ClN₇O₆S
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	SGQDAIFBYPRIDQ-MHZLTWQESA-N
Smiles	ClC1=CC=C(C2=N[C@@H](CC(NCCOCCOCCNC(CCCCCN3C(C=CC3=O)=O)=O)=O)C4=NN=C(C)N4C5=C2C(C)=C(C)S5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for (+)-JQ1 maleimide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	72.43	100

Preparing Stock Solutions for (+)-JQ1 maleimide

The following data is based on the product molecular weight 724.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.38 mL	6.90 mL	13.81 mL
5 mM	0.28 mL	1.38 mL	2.76 mL
10 mM	0.14 mL	0.69 mL	1.38 mL
50 mM	0.03 mL	0.14 mL	0.28 mL

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References for (+)-JQ1 maleimide

References are publications that support the biological activity of the product.

Pinch A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes. Cell Chem.Biol. 2022 PMID: 34499862