PPADS tetrasodium salt

Name: PPADS tetrasodium salt
Brand: Tocris Bioscience
SKU: 0625
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 0625

(2)

Citations (27)

COA / SDS

Non-selective P2 antagonist

Key Product Details

Description:	Non-selective P2 antagonist
Chemical Name:	Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid tetrasodium salt
Molecular Weight:	599.3

View Full Technical Details for PPADS tetrasodium salt

Technical Data
Citations / Reviews (2)

Biological Activity for PPADS tetrasodium salt

PPADS tetrasodium salt is a non-selective P2 purinergic antagonist. Blocks recombinant P2X₁, P2X₂, P2X₃, P2X₅ (IC₅₀ = 1 - 2.6 μM), native P2Y₂-like (IC₅₀ ~ 0.9 mM), and recombinant P2Y₄ (IC₅₀ ~ 15 mM) receptors. PPADS tetrasodium salt delays onset of calcium responses to mild hypoosmotic stress in cortical slices. PPADS tetrasodium salt blocks P2X7 and reduces clinical and histological graft-versus-host disease development in a humanised mouse model.

2'5'-isomer also available.

Technical Data for PPADS tetrasodium salt

M. Wt	599.3
Formula	C₁₄H₁₀N₃Na₄O₁₂PS₂
Storage	Desiccate at -20°C
CAS Number	192575-19-2
PubChem ID	101086948
InChi Key	KURWUCJJNVPCHT-UHFFFAOYSA-J
Smiles	[Na+].[Na+].[Na+].[Na+].CC1=C(O)C(C=O)=C(COP([O-])([O-])=O)C(\N=N\C2=C(C=C(C=C2)S([O-])(=O)=O)S([O-])(=O)=O)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PPADS tetrasodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water		100

Preparing Stock Solutions for PPADS tetrasodium salt

The following data is based on the product molecular weight 599.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.67 mL	8.34 mL	16.69 mL
5 mM	0.33 mL	1.67 mL	3.34 mL
10 mM	0.17 mL	0.83 mL	1.67 mL
50 mM	0.03 mL	0.17 mL	0.33 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for PPADS tetrasodium salt

References are publications that support the biological activity of the product.

Cuthbertson P2X7 receptor antagonism increases regulatory T cells and reduces clinical and histological graft-versus-host disease in a humanised mouse model. Clin.Sci. 2021 PMID: 33463682
Thrane Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema. Proc.Natl.Acad.Sci.U S A 2011 PMID: 21187412
Ralevic and Burnstock Receptors for purines and pyrimidines. Pharmacol.Rev. 1998 PMID: 9755289
McLaren Investigation of the actions of PPADS, a novel P_2x-purinoceptor antagonist, in the guinea-pig isolated vas deferens. Br.J.Pharmacol. 1994 PMID: 8019769
Lambrecht PPADS, a novel functionally selective antagonist of P₂ purinoceptor-mediated responses. Eur.J.Pharmacol. 1992 PMID: 1330591
Ziganshin Selective antagonism by PPADS at P_2x-purinoceptors in rabbit isolated blood vessels. Br.J.Pharmacol. 1994 PMID: 8019770