PSB 10 hydrochloride

Name: PSB 10 hydrochloride
Brand: Tocris Bioscience
SKU: 2010

Tocris Bioscience, part of Bio-Techne | Catalog # 2010

Citations (453)

COA / SDS

Potent, highly selective hA3 receptor antagonist/inverse agonist

Key Product Details

Description:	Potent, highly selective hA₃ receptor antagonist/inverse agonist
Chemical Name:	(8R)-8-Ethyl-1,4,7,8-tetrahydro-4-methyl-2-(2,3,5-trichlorophenyl)-5H-imidazo[2,1-i]purin-5-one monohydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	435.14

View Full Technical Details for PSB 10 hydrochloride

Technical Data
Citations

Biological Activity for PSB 10 hydrochloride

PSB 10 hydrochloride is a potent and highly selective antagonist for the human adenosine A₃ receptor, with low affinity for the rat A₃ receptor (K_i values are 0.44 and > 17000 nM respectively). Displays > 3800-fold selectivity over human A₁, A_2A and A_2B receptors (K_i values are 4.1, 3.3 and 30 μM respectively) and > 1800-fold selectivity over rat A₁ and A_2A receptors. Acts as an inverse agonist in the [³⁵S]GTPγS binding assay in hA₃-CHO cells (IC₅₀ = 4 nM). Produces thermal hyperalgesia in mice in vivo.

Compound Libraries for PSB 10 hydrochloride

PSB 10 hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for PSB 10 hydrochloride

M. Wt	435.14
Formula	C₁₆H₁₄Cl₃N₅O.HCl
Storage	Desiccate at +4°C
Purity	≥98% (HPLC)
CAS Number	591771-91-4
PubChem ID	90488733
InChi Key	JUPMOTHIKGWECW-DDWIOCJRSA-N
Smiles	CC[C@H](C3)N=C1N3C(N(C)C2=C1NC(C4=C(Cl)C(Cl)=CC(Cl)=C4)=N2)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PSB 10 hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	10.88	25
	ethanol	4.35	10

Preparing Stock Solutions for PSB 10 hydrochloride

The following data is based on the product molecular weight 435.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	9.19 mL	45.96 mL	91.92 mL
1.25 mM	1.84 mL	9.19 mL	18.38 mL
2.5 mM	0.92 mL	4.60 mL	9.19 mL
12.5 mM	0.18 mL	0.92 mL	1.84 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for PSB 10 hydrochloride

References are publications that support the biological activity of the product.

Ozola 2-Phenylimidazo[2,1-i]purin-5-ones: structure-activity relationships and characterization of potent and selective inverse agonists at human A₃ adenosine receptors. Bioorg.Med.Chem. 2003 PMID: 12517430
Abo-Salem Antinociceptive effects of novel A_2B adenosine receptor antagonists. J.Pharmacol.Exp.Ther. 2004 PMID: 14563788
Muller Medicinal chemistry of adenosine A₃ receptor ligands. Curr.Top.Med.Chem. 2003 PMID: 12570761