(Z)-Pugnac

Name: ( Z )-Pugnac
Brand: Tocris Bioscience
SKU: 3384
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3384

Citations (24)

COA / SDS

O-GlcNAcase and β-hexosaminidase inhibitor

Key Product Details

Description:	O-GlcNAcase and β-hexosaminidase inhibitor
Chemical Name:	O-(2-Acetamido-2-deoxy-D-glucopyranosylidene)amino-Z-N-phenylcarbamate
Purity:	≥98% (HPLC)
Molecular Weight:	353.33

View Full Technical Details for (Z)-Pugnac

Technical Data
Citations

Biological Activity for (Z)-Pugnac

(Z)-Pugnac is an O-GlcNAc-β-N-acetylglucosaminidase (O-GlcNAcase) and β-hexosaminidase inhibitor (K_i values are 46 and 36 nM respectively) that increases O-GlcNAc levels ~ 2-fold in HT29 cells. Z-linked isomer is more potent than the E isomer.

Technical Data for (Z)-Pugnac

M. Wt	353.33
Formula	C₁₅H₁₉N₃O₇
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	132489-69-1
PubChem ID	6074876
InChi Key	PBLNJFVQMUMOJY-JXAWBTAJSA-N
Smiles	OC(C1NC(C)=O)C(O)C(CO)O/C1=N\OC(NC2=CC=CC=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for (Z)-Pugnac

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	35.33	100

Preparing Stock Solutions for (Z)-Pugnac

The following data is based on the product molecular weight 353.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.83 mL	14.15 mL	28.30 mL
5 mM	0.57 mL	2.83 mL	5.66 mL
10 mM	0.28 mL	1.42 mL	2.83 mL
50 mM	0.06 mL	0.28 mL	0.57 mL

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References for (Z)-Pugnac

References are publications that support the biological activity of the product.

Macauley O-GlcNAcase uses substrate-assisted catalysis. J.Biol.Chem. 2005 PMID: 15795231
Perreira Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry. Bioorg.Med.Chem. 2006 PMID: 16214344
Dong and Hart Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol. J.Biol.Chem. 1994 PMID: 8034696