Raphin 1

Name: Raphin 1
Brand: Tocris Bioscience
SKU: 6760

Tocris Bioscience, part of Bio-Techne | Catalog # 6760

Citations (3)

COA / SDS

Inhibitor of the regulatory subunit PPP1R15B of protein phosphatase 1; orally bioavailable and brain penetrant

Key Product Details

Description:	Inhibitor of the regulatory subunit PPP1R15B of protein phosphatase 1; orally bioavailable and brain penetrant
Chemical Name:	(2E)-2-[(2,3-Dichlorophenyl)methylene]hydrazinecarboximidamide
Purity:	≥98% (HPLC)
Molecular Weight:	231.08

View Full Technical Details for Raphin 1

Technical Data
Citations

Biological Activity for Raphin 1

Raphin 1 is an inhibitor of the regulatory subunit PPP1R15B of protein phosphatase (K_d = 33 nM). Displays >30-fold selectivity for PPP1R15B over PPP1R15A. Inhibits PPP1R15B-mediated dephosphorylation and protein synthesis in vitro. Induces transient reduction in protein synthesis in brains of wild type mice after single dose. Decreases SDS-insoluble huntingtin assemblies and nuclear inclusions in a mouse model of Huntington's disease. Orally bioavailable and brain penetrant.

Compound Libraries for Raphin 1

Raphin 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Raphin 1

M. Wt	231.08
Formula	C₈H₈Cl₂N₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2022961-17-5
PubChem ID	5356739
InChi Key	WLTSTDGGFCQWTK-PQMHYQBVSA-N
Smiles	NC(N/N=C/C1=C(C(Cl)=CC=C1)Cl)=N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Raphin 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	23.11	100
	ethanol	11.55	50

Preparing Stock Solutions for Raphin 1

The following data is based on the product molecular weight 231.08.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.33 mL	21.64 mL	43.28 mL
5 mM	0.87 mL	4.33 mL	8.66 mL
10 mM	0.43 mL	2.16 mL	4.33 mL
50 mM	0.09 mL	0.43 mL	0.87 mL

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References for Raphin 1

References are publications that support the biological activity of the product.

Krzyzosiak Target-based discovery of an inhibitor of the regulatory phosphatase PPP1R15B. Cell. 2018 PMID: 30057111