RO-3

Tocris Bioscience, part of Bio-Techne | Catalog # 3052

COA / SDS

Selective P2X3 and P2X2/3 antagonist

Key Product Details

Description:	Selective P2X₃ and P2X_2/3 antagonist
Chemical Name:	5-[[4,5-Dimethoxy-2-(methylethyl)phenyl]methyl]-2,4-pyrimidinediamine
Purity:	≥98% (HPLC)
Molecular Weight:	302.37

View Full Technical Details for RO-3

Technical Data
Citations

Biological Activity for RO-3

RO-3 is a selective homomeric P2X₃ and heteromeric P2X_2/3 receptor antagonist (pIC₅₀ values are 7.0 and 5.9 respectively) that exhibits no activity at P2X₁, P2X₂, P2X₄, P2X₅ and P2X₇ receptors (IC₅₀ > 10 μM). Attenuates nociceptive sensitivity in animal models of pain. Orally active and brain penetrant.

Technical Data for RO-3

M. Wt	302.37
Formula	C₁₆H₂₂N₄O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	1026582-88-6
PubChem ID	11289644
InChi Key	PYNPWUIBJMVRIG-UHFFFAOYSA-N
Smiles	NC1=NC(N)=NC=C1CC2=C(C(C)C)C=C(OC)C(OC)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RO-3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.24	100

Preparing Stock Solutions for RO-3

The following data is based on the product molecular weight 302.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.31 mL	16.54 mL	33.07 mL
5 mM	0.66 mL	3.31 mL	6.61 mL
10 mM	0.33 mL	1.65 mL	3.31 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for RO-3

References are publications that support the biological activity of the product.

Donnelly-Roberts Painful purinergic receptors. J.Pharmacol.Exp.Ther. 2008 PMID: 18042830
Gever Pharmacology of P2X channels. Eur.J.Physiol.
Ford Purinoceptors as therapeutic targets for lower urinary tract dysfunction. Br.J.Pharmacol. 2006 PMID: 16465177