Ro 5-3335

Name: Ro 5-3335
Brand: Tocris Bioscience
SKU: 4694

Tocris Bioscience, part of Bio-Techne | Catalog # 4694

Citations (9)

COA / SDS

Core binding factor inhibitor; attenuates hematopoiesis

Key Product Details

Description:	Core binding factor inhibitor; attenuates hematopoiesis
Alternative Names:	CBFβ-Runx1 inhibitor II
Chemical Name:	7-Chloro-1,3-dihydro-5-(1H-pyrrol-2-yl)-2H-1,4-benzodiazepin-2-one
Purity:	≥98% (HPLC)
Molecular Weight:	259.69

View Full Technical Details for Ro 5-3335

Technical Data
Citations

Biological Activity for Ro 5-3335

Ro 5-3335 is a core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC₅₀ = 1.1 μM). Represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. Reduces leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. Also a Tat antagonist; inhibits HIV-1 replication in vitro.

Compound Libraries for Ro 5-3335

Ro 5-3335 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for Ro 5-3335

M. Wt	259.69
Formula	C₁₃H₁₀ClN₃O
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	30195-30-3
PubChem ID	64983
InChi Key	XWNMORIHKRROGW-UHFFFAOYSA-N
Smiles	O=C1CN=C(C3=CC=CN3)C2=C(C=CC(Cl)=C2)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ro 5-3335

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.96	100

Preparing Stock Solutions for Ro 5-3335

The following data is based on the product molecular weight 259.69.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.85 mL	19.25 mL	38.51 mL
5 mM	0.77 mL	3.85 mL	7.70 mL
10 mM	0.39 mL	1.93 mL	3.85 mL
50 mM	0.08 mL	0.39 mL	0.77 mL

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References for Ro 5-3335

References are publications that support the biological activity of the product.

Cupelli The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter. J.Virol. 1995 PMID: 7884917
Okuda RUNX1/AML1: a central player in hematopoiesis. Int. J. Hematol. 2001 PMID: 11721959
Cunningham Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF beta interaction. Proc.Natl.Acad.Sci.USA 2012 PMID: 22912405