Ro 5-3335

Name: Ro 5-3335
Brand: Tocris Bioscience
SKU: 4694

Tocris Bioscience, part of Bio-Techne | Catalog # 4694

Citations (9)

COA / SDS

Core binding factor inhibitor; attenuates hematopoiesis

Key Product Details

Chemical Name:	7-Chloro-1,3-dihydro-5-(1H-pyrrol-2-yl)-2H-1,4-benzodiazepin-2-one
Purity:	≥98%

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Technical Data
Citations

Biological Activity for Ro 5-3335

Ro 5-3335 is a core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC₅₀ = 1.1 μM). Represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. Reduces leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. Also a Tat antagonist; inhibits HIV-1 replication in vitro.

Technical Data for Ro 5-3335

Formula	C₁₃H₁₀ClN₃O
Storage	Store at +4°C
Purity	≥98%
CAS Number	30195-30-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ro 5-3335

Solubility

Soluble Soluble to 100 mM in DMSO

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References for Ro 5-3335

References are publications that support the biological activity of the product.

T Huang, M Yang, K Dong, M Xu, J Liu, Z Chen, S Zhu, W Chen, J Yin, K Jin, Y Deng, Z Guan, X Huang, J Yang, R Han, M Yao A transcriptional landscape of 28 porcine tissues obtained by super deepSAGE sequencing BMC Genomics, 2020;21(1):229. 2020 PMID: 32171242
Cupelli et al. The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter. J.Virol., 1995;69:2640 PMID: 7884917
Cunningham et al. Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF� interaction. Proc.Natl.Acad.Sci.USA, 2012;109:14592 PMID: 22912405
Okuda et al. RUNX1/AML1: a central player in hematopoiesis. Int. J. Hematol., 2001;74:252 PMID: 11721959

Ro 5-3335

Tocris Bioscience, part of Bio-Techne | Catalog # 4694

Key Product Details

Biological Activity for Ro 5-3335

Technical Data for Ro 5-3335

Solubility for Ro 5-3335

Dilution Calculator

Reconstitution Calculator

References for Ro 5-3335

Product Documents for Ro 5-3335

COA

Certificate of Analysis

SDS

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